BRENDA - Enzyme Database
show all sequences of 4.4.1.22

A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy

Gönrich, M.; Bartoschek, S.; Hagemeier, C.H.; Griesinger, C.; Vorholt, J.A.; J. Biol. Chem. 277, 3069-3072 (2002)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
x * 21000, SDS-PAGE
Paracoccus denitrificans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-(hydroxymethyl)glutathione
Paracoccus denitrificans
the enzyme accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway
glutathione + formaldehyde
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paracoccus denitrificans
-
-
-
Purification (Commentary)
Commentary
Organism
-
Paracoccus denitrificans
Reaction
Reaction
Commentary
Organism
S-(hydroxymethyl)glutathione = glutathione + formaldehyde
the enzyme from Paracoccus denitrificans accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway
Paracoccus denitrificans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
350
-
-
Paracoccus denitrificans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-(hydroxymethyl)glutathione
-
656068
Paracoccus denitrificans
glutathione + formaldehyde
-
-
-
?
S-(hydroxymethyl)glutathione
the enzyme accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway
656068
Paracoccus denitrificans
glutathione + formaldehyde
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 21000, SDS-PAGE
Paracoccus denitrificans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
x * 21000, SDS-PAGE
Paracoccus denitrificans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-(hydroxymethyl)glutathione
Paracoccus denitrificans
the enzyme accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway
glutathione + formaldehyde
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Paracoccus denitrificans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
350
-
-
Paracoccus denitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-(hydroxymethyl)glutathione
-
656068
Paracoccus denitrificans
glutathione + formaldehyde
-
-
-
?
S-(hydroxymethyl)glutathione
the enzyme accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway
656068
Paracoccus denitrificans
glutathione + formaldehyde
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 21000, SDS-PAGE
Paracoccus denitrificans
Other publictions for EC 4.4.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749071
Hopkinson
Studies on the glutathione-de ...
Paracoccus denitrificans
PLoS ONE
10
e0145085
2015
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1
1
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1
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1
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4
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1
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1
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1
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1
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1
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1
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2
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2
2
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665643
Neculai
A dynamic zinc redox switch ...
Paracoccus denitrificans
J. Biol. Chem.
280
2826-2830
2005
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1
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1
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2
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1
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1
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1
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1
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1
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1
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-
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-
656068
Gönrich
A glutathione-dependent formal ...
Paracoccus denitrificans
J. Biol. Chem.
277
3069-3072
2002
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-
-
-
-
-
-
-
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1
1
-
6
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1
1
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1
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2
1
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1
1
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1
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1
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2
1
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