Protein Variants | Comment | Organism |
---|---|---|
C83A | mutant shows lower activity than the wild type enzyme | Vibrio harveyi |
E57A | mutant shows lower activity than the wild type enzyme | Vibrio harveyi |
E57D | mutant shows lower activity than the wild type enzyme | Vibrio harveyi |
H11Q | mutant shows lower activity than the wild type enzyme | Vibrio harveyi |
R39M | mutant shows lower activity than the wild type enzyme | Vibrio harveyi |
S6A | mutant shows lower activity than the wild type enzyme | Vibrio harveyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | - |
Bacillus subtilis | |
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | - |
Escherichia coli | |
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | - |
Vibrio harveyi | |
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | - |
Bacillus subtilis | |
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | - |
Escherichia coli | |
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | - |
Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
S-ribosylhomocysteine | wild type enzyme | Bacillus subtilis | |
0.0036 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | wild type enzyme | Bacillus subtilis | |
0.027 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | wild type enzyme | Escherichia coli | |
0.027 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | wild type enzyme | Vibrio harveyi | |
0.037 | - |
S-ribosylhomocysteine | wild type enzyme | Escherichia coli | |
0.037 | - |
S-ribosylhomocysteine | wild type enzyme | Vibrio harveyi | |
0.041 | - |
S-ribosylhomocysteine | mutant enzyme S6A | Vibrio harveyi | |
0.041 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme S6A | Vibrio harveyi | |
0.098 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme E57A | Vibrio harveyi | |
0.15 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme R39M | Vibrio harveyi | |
0.52 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme H11Q | Vibrio harveyi | |
0.7 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme C83A | Vibrio harveyi | |
0.73 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme E57D | Vibrio harveyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site | Bacillus subtilis | |
Fe2+ | LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site | Escherichia coli | |
Fe2+ | LuxS is a metalloenzyme containing a tetrahedrally coordinated Fe2+ ion in its active site | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Vibrio harveyi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | - |
Bacillus subtilis | L-homocysteine + ? | - |
? | |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | - |
Escherichia coli | L-homocysteine + ? | - |
? | |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | - |
Vibrio harveyi | L-homocysteine + ? | - |
? | |
S-ribosylhomocysteine | - |
Bacillus subtilis | L-homocysteine + 4,5-dihydroxy-2,3-pentanedione | - |
? | |
S-ribosylhomocysteine | - |
Escherichia coli | L-homocysteine + 4,5-dihydroxy-2,3-pentanedione | - |
? | |
S-ribosylhomocysteine | - |
Vibrio harveyi | L-homocysteine + 4,5-dihydroxy-2,3-pentanedione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LuxS | - |
Bacillus subtilis |
LuxS | - |
Escherichia coli |
LuxS | - |
Vibrio harveyi |
S-ribosylhomocysteinase | - |
Bacillus subtilis |
S-ribosylhomocysteinase | - |
Escherichia coli |
S-ribosylhomocysteinase | - |
Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme E57A | Vibrio harveyi | |
0.016 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme R39M | Vibrio harveyi | |
0.022 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme C83A | Vibrio harveyi | |
0.024 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme H11Q | Vibrio harveyi | |
0.026 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme E57D | Vibrio harveyi | |
0.027 | - |
S-ribosylhomocysteine | mutant enzyme S6A | Vibrio harveyi | |
0.03 | - |
S-ribosylhomocysteine | wild type enzyme | Bacillus subtilis | |
0.038 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | wild type enzyme | Bacillus subtilis | |
0.14 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | mutant enzyme S6A | Vibrio harveyi | |
0.4 | - |
S-ribosylhomocysteine | wild type enzyme | Escherichia coli | |
0.4 | - |
S-ribosylhomocysteine | wild type enzyme | Vibrio harveyi | |
0.4 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | wild type enzyme | Escherichia coli | |
0.64 | - |
(2R)-2-amino-4-[[(2S,4S)-2,4,5-trihydroxy-3-oxopentyl]sulfanyl]butanoic acid | wild type enzyme | Vibrio harveyi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0079 | - |
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | wild type enzyme | Bacillus subtilis | |
0.0106 | - |
S-[3-fluoro-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | wild type enzyme | Bacillus subtilis | |
0.047 | - |
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | wild type enzyme | Escherichia coli | |
0.047 | - |
S-[3-bromo-3,5-dideoxy-D-ribofuranos-5-yl]-L-homocysteine | wild type enzyme | Vibrio harveyi |