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Literature summary for 4.4.1.21 extracted from

  • Rajan, R.; Zhu, J.; Hu, X.; Pei, D.; Bell, C.E.
    Crystal structure of S-ribosylhomocysteinase (LuxS) in complex with a catalytic 2-ketone intermediate (2005), Biochemistry, 44, 3745-3753.
    View publication on PubMed
Search for more literature for 4.4.1.21

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion, inactive mutant C84A of Co2+-substituted LuxS is cocrystallized with the 2-ketone intermediate and the structure is determined to 1.8 A resolution Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C85A catalytically inactive mutant Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 S-ribosylhomocysteine
-
 Bacillus subtilis
0.039
-
 S-ribosylhomocysteine
-
 Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34667
-
-
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio harveyi
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ribosylhomocysteine
-
 Vibrio harveyi L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?
S-ribosylhomocysteine
-
 Bacillus subtilis L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 S-ribosylhomocysteine
-
 Bacillus subtilis
0.4
-
 S-ribosylhomocysteine
-
 Vibrio harveyi