Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
the interaction of residue R104 with the thiol group of GSH reduces its pKa to allow formation of a thiolate anion and subsequent nucleophilic attack at C6 of LTA4.Crystal structure of mutant R31Q at 2.1 A reveals a Q31 side chain pointing away from the active site | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R104A | 5.7% of wild-type activity | Homo sapiens |
R104K | 0.1% of wild-type activity | Homo sapiens |
R104S | 4.6% of wild-type activity | Homo sapiens |
R104T | 1.1% of wild-type activity | Homo sapiens |
R31A | 12% of wild-type activity | Homo sapiens |
R31Q | 30% of wild-type activity | Homo sapiens |
R51A | 57% of wild-type activity | Homo sapiens |
R51Q | 29% of wild-type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
leukotriene A4 | mutant R31A, 22°C, pH not specified in the publication | Homo sapiens | |
0.07 | - |
leukotriene A4 | mutant R31Q, 22°C, pH not specified in the publication | Homo sapiens | |
0.1 | - |
leukotriene A4 | mutant R104S, 22°C, pH not specified in the publication | Homo sapiens | |
0.3 | - |
leukotriene A4 | wild-type, 22°C, pH not specified in the publication | Homo sapiens | |
0.5 | - |
leukotriene A4 | mutant R104A, 22°C, pH not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16873 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.044 | - |
mutant R104K, 22°C, pH not specified in the publication | Homo sapiens |
0.4 | - |
mutant R104T, 22°C, pH not specified in the publication | Homo sapiens |
1.6 | - |
mutant R104S, 22°C, pH not specified in the publication | Homo sapiens |
2 | - |
mutant R104A, 22°C, pH not specified in the publication | Homo sapiens |
4.3 | - |
mutant R31A, 22°C, pH not specified in the publication | Homo sapiens |
10 | - |
mutant R51Q, 22°C, pH not specified in the publication | Homo sapiens |
10.5 | - |
mutant R31Q, 22°C, pH not specified in the publication | Homo sapiens |
20 | - |
mutant R51A, 22°C, pH not specified in the publication | Homo sapiens |
35 | - |
wild-type, 22°C, pH not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + glutathione | - |
Homo sapiens | leukotriene C4 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
leukotriene A4 | mutant R104S, 22°C, pH not specified in the publication | Homo sapiens | |
0.66 | - |
leukotriene A4 | mutant R104A, 22°C, pH not specified in the publication | Homo sapiens | |
1.4 | - |
leukotriene A4 | mutant R31A, 22°C, pH not specified in the publication | Homo sapiens | |
1.8 | - |
leukotriene A4 | mutant R31Q, 22°C, pH not specified in the publication | Homo sapiens | |
11.7 | - |
leukotriene A4 | wild-type, 22°C, pH not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
leukotriene A4 | mutant R104A, 22°C, pH not specified in the publication | Homo sapiens | |
5 | - |
leukotriene A4 | mutant R104S, 22°C, pH not specified in the publication | Homo sapiens | |
26 | - |
leukotriene A4 | mutant R31Q, 22°C, pH not specified in the publication | Homo sapiens | |
28 | - |
leukotriene A4 | mutant R31A, 22°C, pH not specified in the publication | Homo sapiens | |
39 | - |
leukotriene A4 | wild-type, 22°C, pH not specified in the publication | Homo sapiens |