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Literature summary for 4.4.1.20 extracted from

  • Wetterholm, A.; Martinez Molina, D.; Nordlund, P.; Eshaghi, S.; Haeggstroem, J.Z.
    High-level expression, purification, and crystallization of recombinant rat leukotriene C4 synthase from the yeast Pichia pastoris (2008), Protein Expr. Purif., 60, 1-6.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as an N-terminal hexa-histidine-tagged fusion protein in Pichia pastoris KM71H cells Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method using the MbClass and MbClass II suites Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, S-hexylglutathione agarose chromatography, and Superdex 200 gel filtration Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.29
-
crude enzyme from homogenate, at 25°C Rattus norvegicus
28
-
recombinant enzyme, after 97fold purification, at 25°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + glutathione
-
Rattus norvegicus leukotriene C4 + H2O
-
?

Synonyms

Synonyms Comment Organism
leukotriene C4 synthase
-
Rattus norvegicus
LTC4S
-
Rattus norvegicus