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Literature summary for 4.4.1.20 extracted from

  • Lam, B.K.; Frank Austen, K.
    Leukotriene C4 synthase: a pivotal enzyme in cellular biosynthesis of the cysteinyl leukotrienes (2002), Prostaglandins Other Lipid Mediat., 68-69, 511-520.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A52S mutation increases the Km-value for the recombinant enzyme for glutathione Homo sapiens
C56S mutant enzyme without altered function Homo sapiens
C56S/C82 mutant enzyme without altered function Homo sapiens
C82V mutant enzyme without altered function Homo sapiens
N55A mutation increases the Km-value for the recombinant enzyme for glutathione Homo sapiens
R51H fully active mutant enzyme Homo sapiens
R51I mutant enzyme without activity Homo sapiens
R51K fully active mutant enzyme Homo sapiens
R51T mutant enzyme without activity Homo sapiens
V49F mutation increases the Km-value for the recombinant enzyme for glutathione Homo sapiens
Y59F mutation increases the Km-value for the recombinant enzyme for glutathione Homo sapiens
Y93F activity of the mutant enzyme is less than 1% of the wild-type enzyme, shift in pH optimum of the residual activity to that of spontaneous conjugation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NEM
-
Cavia porcellus
NEM
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mg2+ enhances activity Mus musculus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + glutathione
-
Cavia porcellus leukotriene C4 + H2O
-
?
leukotriene A4 + glutathione
-
Mus musculus leukotriene C4 + H2O
-
?
leukotriene A4 + glutathione
-
Homo sapiens leukotriene C4 + H2O
-
?
leukotriene A4 + glutathione
-
Rattus norvegicus leukotriene C4 + H2O
-
?