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Literature summary for 4.4.1.20 extracted from
- Solubilization and partial purification of leukotriene C4 synthase from guinea-pig lung: a microsomal enzyme with high specificity towards 5,6-epoxide leukotriene A4 (1988), Biochim. Biophys. Acta, 959, 305-315.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hexylglutathione | IC50: 1.4 mM | Cavia porcellus |  |
| hexylglutathione | - |
Rattus norvegicus | |
| NEM | IC50: 0.018 mM | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
leukotriene A4 methyl ester | pH 8.0, 37°C | Cavia porcellus | |
| 0.036 | - |
leukotriene A4 | pH 8.0, 37°C | Cavia porcellus | |
| 0.07 | - |
14,15-Leukotriene A4 methyl ester | pH 8.0, 37°C | Cavia porcellus | |
| 0.13 | - |
14,15-Leukotriene A4 | pH 8.0, 37°C | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Cavia porcellus | - |
- |
| microsome | - |
Rattus norvegicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | low activity | Cavia porcellus | - |
| brain | low activity | Cavia porcellus | - |
| colon | low activity | Cavia porcellus | - |
| heart | - |
Cavia porcellus | - |
| kidney | - |
Cavia porcellus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| liver | low activity | Cavia porcellus | - |
| lung | - |
Cavia porcellus | - |
| lung | - |
Rattus norvegicus | - |
| small intestine | low activity | Cavia porcellus | - |
| spleen | - |
Cavia porcellus | - |
| stomach | low activity | Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00786 | - |
- |
Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 14,15-leukotriene A4 + glutathione | - |
Cavia porcellus | ? | - |
? | |
| 14,15-leukotriene A4 methyl ester + glutathione | - |
Cavia porcellus | ? | - |
? | |
| leukotriene A4 + glutathione | - |
Cavia porcellus | leukotriene C4 + H2O | - |
? | |
| leukotriene A4 + glutathione | - |
Rattus norvegicus | leukotriene C4 + H2O | - |
? | |
| leukotriene A4 methyl ester + glutathione | - |
Cavia porcellus | leukotriene C4 methyl ester + H2O | - |
? | |
| leukotriene A4 methyl ester + glutathione | - |
Rattus norvegicus | leukotriene C4 methyl ester + H2O | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
3 min, 50 min loss of activity, 10 mM glutathione completely protects against inactivation | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8.2 | - |
Cavia porcellus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
IC50: 0.018 mM | Cavia porcellus | NEM | |
| 1.4 | - |
IC50: 1.4 mM | Cavia porcellus | hexylglutathione | |
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