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Literature summary for 4.4.1.17 extracted from

  • Zhang, Y.; Stevens, J.M.; Ferguson, S.J.
    Substrate recognition of holocytochrome c synthase: N-terminal region and CXXCH motif of mitochondrial cytochrome c (2014), FEBS Lett., 588, 3367-3374.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.17

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C15A site-directed mutagenesis, cytoplasm-targeted mutant, the mutant is matured and undergoes heme attachment like the wild-type enzyme Saccharomyces cerevisiae
C18A site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
F15A site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c Saccharomyces cerevisiae
F15E site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c Saccharomyces cerevisiae
F15I site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme Saccharomyces cerevisiae
F15W site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme Saccharomyces cerevisiae
F15Y site-directed mutagenesis, periplasm-targeted mutant, the mutant shows unaltered holocytochrome c production like the wild-type enzyme Saccharomyces cerevisiae
H19K site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
H19M site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
H19R site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
additional information construction of T17, K16, G29, H45, and K60 deletion mutants and of a mutant with periplasmic targeting sequence Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Apocytochrome c + heme Saccharomyces cerevisiae
-
 Holocytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Apocytochrome c + heme
-
 Saccharomyces cerevisiae Holocytochrome c
-
 ?
Apocytochrome c + heme Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiae cytochrome c are coexpressed in Escherichia coli strain BL21(DE3) with Saccharomyces cerevisiae holocytochrome c synthase. No product synthesis from truncated cytochrome c1 mutants G29X, H45X and K60X, with the X indicating the position of the inserted stop codon Saccharomyces cerevisiae Holocytochrome c
-
 ?
additional information the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important protein-protein recognition motifs in enzyme-substrate interaction, the N-terminal region of the mitochondrial cytochrome c sequence is critical for attachment of heme to apocytochrome c Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
HCC synthase
-
 Saccharomyces cerevisiae
HCCS
-
 Saccharomyces cerevisiae
holocytochrome c synthase
-
 Saccharomyces cerevisiae