Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D146K | site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity | Homo sapiens |
D146K/H389T | site-directed mutagenesis,the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity to a higher extent | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | Homo sapiens | - |
selenide + L-alanine + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96I15 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by tag cleavage through TEV protease, another step of nickel affinity chromatography, and dialysis | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor | reaction mechanism and mechanism of substrate recognition, model, overview. A pyridoxal 5'-phosphate-substrate intermediate is attacked by a deprotonated active site Cys, producing the protein-bound persulfide or sulfoselenide, formation of the external Cys-aldimine intermediate. | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | - |
Homo sapiens | selenide + L-alanine + acceptor | - |
? | |
additional information | the enzyme is specific for L-selenocysteine versus L-cysteine, substrate specificity for selenium and sulfur is determined by a single residue, Asp246, in human selenocysteine lyase, chemical basis for and mechanism of the enzyme's substrate discrimination of wild-type enzyme and mutant D146K/H389T, overview. The wild-type reaction with Cys is a single-turnover reaction, producing a dead-end protein adduct | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCL | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |