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Literature summary for 4.4.1.14 extracted from

  • Wang, H.; Mei, W.; Qin, Y.; Zhu, Y.
    1-Aminocyclopropane-1-carboxylic acid synthase 2 is phosphorylated by calcium-dependent protein kinase 1 during cotton fiber elongation (2011), Acta Biochim. Biophys. Sin., 43, 654-661.
    View publication on PubMed
Search for more literature for 4.4.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gossypium hirsutum

Protein Variants

Protein Variants Comment Organism
S460G level of CPK1 phosphorylation is significantly decreased Gossypium hirsutum
S460G/S478A/S481A/S486A level of CPK1 phosphorylation is significantly decreased Gossypium hirsutum
S478A/S481A/S486A CPK1 phosphorylation efficiency does not significantly change compared to wild-type Gossypium hirsutum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, SDS-PAGE, x * 57900, calculated Gossypium hirsutum
57900
-
 x * 57000, SDS-PAGE, x * 57900, calculated Gossypium hirsutum

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum A2IBN7 cv Xuzhou 142, isoform ACS2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein cotton ACS2 interacts with Ca2+-dependent protein kinase CPK1. Bacterially expressed and purified recombinant ACS2 is phosphorylated by CPK1 in vitro and residue S460 is a possible phosphorylation site for CPK1. Phosphorylated ACS2 shows significantly increased ACS activity, leading to elevated ethylene production Gossypium hirsutum

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Gossypium hirsutum

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.85
-
 pH 7.5, 30°C, native enzyme Gossypium hirsutum
2.78
-
 pH 7.5, 30°C, enzyme phosphorylated by CPK1 Gossypium hirsutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
 Gossypium hirsutum 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE, x * 57900, calculated Gossypium hirsutum

General Information

General Information Comment Organism
physiological function cotton ACS2 interacts with Ca2+-dependent protein kinase CPK1. Phosphorylated ACS2 shows significantly increased ACS activity, leading to elevated ethylene production Gossypium hirsutum