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Literature summary for 4.4.1.14 extracted from

  • Li, J.F.; Qu, L.H.; Li, N.
    Tyr152 plays a central role in the catalysis of 1-aminocyclopropane-1-carboxylate synthase (2005), J. Exp. Bot., 56, 2203-2210.
    View publication on PubMed
Search for more literature for 4.4.1.14

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of isoenzyme LeACS2 and five ACC synthase mutants (Y151F, Y151G, Y152F, Y152G and Y151F/Y152F) in Escherichia coli Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
Y151F activity is reduced by 27% Solanum lycopersicum
Y151F/Y152F activity is reduced by 99% Solanum lycopersicum
Y151G activity is reduced by 83% Solanum lycopersicum
Y152F activity is reduced by 98% Solanum lycopersicum
Y152G inactive mutant enzyme Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0315
-
 S-adenosyl-L-methionine 30°C, pH 8.5, wild-type enzyme Solanum lycopersicum
0.0458
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F Solanum lycopersicum
0.168
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151G Solanum lycopersicum
0.409
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F/Y152F Solanum lycopersicum
0.514
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y152F Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum key enzyme in the regulation of ethylene biosynthesis in higher plants ?
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme LeACS2 and five ACC synthase mutants (Y151F, Y151G, Y152F, Y152G and Y151F/Y152F) in Escherichia coli Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information key enzyme in the regulation of ethylene biosynthesis in higher plants Solanum lycopersicum ?
-
 ?
S-adenosyl-L-methionine
-
 Solanum lycopersicum 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Synonyms

Synonyms Comment Organism
LeACS2
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F/Y152F Solanum lycopersicum
0.39
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y152F Solanum lycopersicum
1.3
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151G Solanum lycopersicum
1.41
-
 S-adenosyl-L-methionine 30°C, pH 8.5, wild-type enzyme Solanum lycopersicum
1.5
-
 S-adenosyl-L-methionine 30°C, pH 8.5, mutant enzyme Y151F Solanum lycopersicum