Application | Comment | Organism |
---|---|---|
medicine | covalent binding of enzyme to polyethylene glycol moieties reduces the specific activity from 59.71 U/mg of free enzyme to 48.71 U/mg, with a PEGylation yield of 81.5 and 70.7% modification of surface epsilon-amino groups. The pH stability does not change upon modification, and at 50 ° C, the thermal stability of PEG-CGL is increased by 40% in comparison to free CGL. By in vitro proteolysis, PEG-CGL retains more than 50% of its initial activity compared to less than 10% of the free-CGL for acid protease for 30 min. The biochemical and hematological responses of rabbits indicate nil toxicity of free and PEG-CGL | Aspergillus carneus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxonorleucine | - |
Aspergillus carneus | |
iodoacetate | - |
Aspergillus carneus | |
propargylglycine | - |
Aspergillus carneus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.36 | - |
DL-cystathionine | native enzyme, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | |
3.4 | - |
DL-cystathionine | protein bound to polyethylene glycol, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
204000 | - |
PAGE | Aspergillus carneus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus carneus | - |
- |
- |
Aspergillus carneus KF723837 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification by various chromatographic approaches | Aspergillus carneus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.71 | - |
protein bound to polyethylene glycol, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus |
59.71 | - |
native enzyme, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-cystathionine + H2O | - |
Aspergillus carneus | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
DL-cystathionine + H2O | - |
Aspergillus carneus KF723837 | L-cysteine + 2-oxobutanoate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 52000, SDS-PAGE | Aspergillus carneus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life of free enzyme 5.31 h, of PEG-CGL 9.01 h, respectively | Aspergillus carneus |
40 | - |
half-life of free enzyme 2.78 h, of PEG-CGL 3.1 h, respectively | Aspergillus carneus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
DL-cystathionine | protein bound to polyethylene glycol, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | |
0.13 | - |
DL-cystathionine | native enzyme, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.66 | - |
protein bound to polyethylene glycol, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | iodoacetate | |
0.91 | - |
native enzyme, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | propargylglycine | |
0.98 | - |
native enzyme, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | iodoacetate | |
1.34 | - |
protein bound to polyethylene glycol, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | 6-diazo-5-oxonorleucine | |
1.49 | - |
protein bound to polyethylene glycol, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | propargylglycine | |
2.16 | - |
native enzyme, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | 6-diazo-5-oxonorleucine |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
DL-cystathionine | protein bound to polyethylene glycol, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus | |
0.04 | - |
DL-cystathionine | native enzyme, pH not specified in the publication, temperature not specified in the publication | Aspergillus carneus |