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Literature summary for 4.4.1.1 extracted from

  • Maresi, E.; Janson, G.; Fruncillo, S.; Paiardini, A.; Vallone, R.; Dominici, P.; Astegno, A.
    Functional characterization and structure-guided mutational analysis of the transsulfuration enzyme cystathionine gamma-lyase from Toxoplasma gondii (2018), Int. J. Mol. Sci., 19, 2111 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of the external aldimine of wild-type in complex with L-cystathionine Toxoplasma gondii

Protein Variants

Protein Variants Comment Organism
N360S mutation does not alter enzyme conformation, but leads to a 56fold decrease in catalyic efficiency for L-cystathionine Toxoplasma gondii
S77A kinetics are similar to wild-type Toxoplasma gondii
S77E complete loss of activity with cystathionine Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
L-cysteine wild-type, pH 9.0, 40°C Toxoplasma gondii
0.4
-
L-cysteine mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.51
-
djenkolic acid wild-type, pH 9.0, 40°C Toxoplasma gondii
0.86
-
djenkolic acid mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.9
-
L-cystathionine wild-type, pH 9.0, 40°C Toxoplasma gondii
1.5
-
djenkolic acid mutant S77A, pH 9.0, 40°C Toxoplasma gondii
2.4
-
L-cystathionine mutant S77A, pH 9.0, 40°C Toxoplasma gondii
6.1
-
L-cystathionine mutant N360S, pH 9.0, 40°C Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
gel filtration Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii B6K8Y1
-
-
Toxoplasma gondii ATCC 50861 B6K8Y1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
djenkolic acid
-
Toxoplasma gondii ?
-
?
djenkolic acid
-
Toxoplasma gondii ATCC 50861 ?
-
?
L-cystathionine + H2O
-
Toxoplasma gondii L-cysteine + 2-oxobutanoate + NH3
-
?
L-cystathionine + H2O
-
Toxoplasma gondii ATCC 50861 L-cysteine + 2-oxobutanoate + NH3
-
?
L-cysteine poor substrate Toxoplasma gondii H2S + NH3 + pyruvate
-
?
L-cysteine poor substrate Toxoplasma gondii ATCC 50861 H2S + NH3 + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 46000, calculated from sequence Toxoplasma gondii

Synonyms

Synonyms Comment Organism
TGME49_112930
-
Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Toxoplasma gondii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
melting temeprature, apoenzyme Toxoplasma gondii
71
-
melting temperature, holoenzyme Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
L-cysteine mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.009
-
L-cysteine wild-type, pH 9.0, 40°C Toxoplasma gondii
0.19
-
djenkolic acid mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.22
-
djenkolic acid mutant S77A, pH 9.0, 40°C Toxoplasma gondii
0.24
-
L-cystathionine mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.24
-
djenkolic acid wild-type, pH 9.0, 40°C Toxoplasma gondii
0.7
-
L-cystathionine mutant S77A, pH 9.0, 40°C Toxoplasma gondii
2
-
L-cystathionine wild-type, pH 9.0, 40°C Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Toxoplasma gondii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate presence of cofactor increases the thermal stability Toxoplasma gondii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.018
-
L-cysteine mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.039
-
L-cystathionine mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.043
-
L-cysteine wild-type, pH 9.0, 40°C Toxoplasma gondii
0.15
-
djenkolic acid mutant S77A, pH 9.0, 40°C Toxoplasma gondii
0.22
-
djenkolic acid mutant N360S, pH 9.0, 40°C Toxoplasma gondii
0.29
-
L-cystathionine mutant S77A, pH 9.0, 40°C Toxoplasma gondii
0.47
-
djenkolic acid wild-type, pH 9.0, 40°C Toxoplasma gondii
2.2
-
L-cystathionine wild-type, pH 9.0, 40°C Toxoplasma gondii