Crystallization (Comment) | Organism |
---|---|
molecular modeling of the external aldimine of wild-type in complex with L-cystathionine | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
N360S | mutation does not alter enzyme conformation, but leads to a 56fold decrease in catalyic efficiency for L-cystathionine | Toxoplasma gondii |
S77A | kinetics are similar to wild-type | Toxoplasma gondii |
S77E | complete loss of activity with cystathionine | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
L-cysteine | wild-type, pH 9.0, 40°C | Toxoplasma gondii | |
0.4 | - |
L-cysteine | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.51 | - |
djenkolic acid | wild-type, pH 9.0, 40°C | Toxoplasma gondii | |
0.86 | - |
djenkolic acid | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.9 | - |
L-cystathionine | wild-type, pH 9.0, 40°C | Toxoplasma gondii | |
1.5 | - |
djenkolic acid | mutant S77A, pH 9.0, 40°C | Toxoplasma gondii | |
2.4 | - |
L-cystathionine | mutant S77A, pH 9.0, 40°C | Toxoplasma gondii | |
6.1 | - |
L-cystathionine | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
gel filtration | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | B6K8Y1 | - |
- |
Toxoplasma gondii ATCC 50861 | B6K8Y1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
djenkolic acid | - |
Toxoplasma gondii | ? | - |
? | |
djenkolic acid | - |
Toxoplasma gondii ATCC 50861 | ? | - |
? | |
L-cystathionine + H2O | - |
Toxoplasma gondii | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cystathionine + H2O | - |
Toxoplasma gondii ATCC 50861 | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cysteine | poor substrate | Toxoplasma gondii | H2S + NH3 + pyruvate | - |
? | |
L-cysteine | poor substrate | Toxoplasma gondii ATCC 50861 | H2S + NH3 + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46000, calculated from sequence | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
TGME49_112930 | - |
Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Toxoplasma gondii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
melting temeprature, apoenzyme | Toxoplasma gondii |
71 | - |
melting temperature, holoenzyme | Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
L-cysteine | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.009 | - |
L-cysteine | wild-type, pH 9.0, 40°C | Toxoplasma gondii | |
0.19 | - |
djenkolic acid | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.22 | - |
djenkolic acid | mutant S77A, pH 9.0, 40°C | Toxoplasma gondii | |
0.24 | - |
L-cystathionine | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.24 | - |
djenkolic acid | wild-type, pH 9.0, 40°C | Toxoplasma gondii | |
0.7 | - |
L-cystathionine | mutant S77A, pH 9.0, 40°C | Toxoplasma gondii | |
2 | - |
L-cystathionine | wild-type, pH 9.0, 40°C | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Toxoplasma gondii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | presence of cofactor increases the thermal stability | Toxoplasma gondii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
L-cysteine | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.039 | - |
L-cystathionine | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.043 | - |
L-cysteine | wild-type, pH 9.0, 40°C | Toxoplasma gondii | |
0.15 | - |
djenkolic acid | mutant S77A, pH 9.0, 40°C | Toxoplasma gondii | |
0.22 | - |
djenkolic acid | mutant N360S, pH 9.0, 40°C | Toxoplasma gondii | |
0.29 | - |
L-cystathionine | mutant S77A, pH 9.0, 40°C | Toxoplasma gondii | |
0.47 | - |
djenkolic acid | wild-type, pH 9.0, 40°C | Toxoplasma gondii | |
2.2 | - |
L-cystathionine | wild-type, pH 9.0, 40°C | Toxoplasma gondii |