Cloned (Comment) | Organism |
---|---|
gene LmjF35.3230 or Lmj_CGL, functional complementation of a CGL null mutant strain DELTA CYS3 of Saccharomyces cerevisiae | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-propargylglycine | an irreversible enzyme inhibitor, molecular mechanism of DL-propargylglycine action in vivo | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania mexicana | 5829 | - |
cytosol | - |
Leishmania major | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | Leishmania mexicana | - |
L-cysteine + 2-oxobutanoate + NH3 | - |
r | |
L-cystathionine + H2O | Leishmania major | - |
L-cysteine + 2-oxobutanoate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | E9AFE7 | gene LmjF35.3230 or Lmj_CGL | - |
Leishmania mexicana | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania mexicana | - |
promastigote | - |
Leishmania major | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.49 | - |
cell-free extract of promastigotes, pH 8.0, 37°C | Leishmania mexicana |
0.49 | - |
cell-free extract of promastigotes, pH 8.0, 37°C | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Leishmania mexicana | L-cysteine + 2-oxobutanoate + NH3 | - |
r | |
L-cystathionine + H2O | - |
Leishmania major | L-cysteine + 2-oxobutanoate + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cgl | - |
Leishmania mexicana |
cgl | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania mexicana |
37 | - |
assay at | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Leishmania mexicana |
8 | - |
assay at | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Leishmania mexicana | |
pyridoxal 5'-phosphate | - |
Leishmania major |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.065 | - |
pH 7.4, 37°C | Leishmania major | DL-propargylglycine |
General Information | Comment | Organism |
---|---|---|
additional information | the Leishmania major enzyme is able to rescue the wild-type phenotype of a Saccharomyces cerevisiae enzyme-null mutant | Leishmania major |