Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D187A | 0.5% of wild-type activity | Homo sapiens |
D187A | the mutation results in a loss of enzyme activity | Homo sapiens |
E339A | the mutant demonstrates an approximately 6fold enhancement in H2S production | Homo sapiens |
E339A | about 6fold enhancement in H2S production | Homo sapiens |
E339K | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
E339Y | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Homo sapiens |
E349A | activity similar to wild-type | Homo sapiens |
E349A | the mutant displays enzyme activity comparable to that for wild type enzyme | Homo sapiens |
K212A | the mutation results in a loss of enzyme activity | Homo sapiens |
K212A | 1.3% of wild-type activity, residue involved in binding of pyridoxal 5'-phosphate | Homo sapiens |
R375A | 2% of wild-type activity | Homo sapiens |
R375A | the mutation results in a loss of enzyme activity | Homo sapiens |
R62A | 3% of wild-type activity | Homo sapiens |
R62A | the mutation results in a loss of enzyme activity | Homo sapiens |
S209A | activity similar to wild-type | Homo sapiens |
S209A | the mutant displays enzyme activity comparable to that for wild type enzyme | Homo sapiens |
T189A | 0.5% of wild-type activity | Homo sapiens |
T189A | the mutation results in a loss of enzyme activity | Homo sapiens |
T211A | activity similar to wild-type | Homo sapiens |
T211A | the mutant displays enzyme activity comparable to that for wild type enzyme | Homo sapiens |
Y114A | the mutation results in a loss of enzyme activity | Homo sapiens |
Y114A | 3% of wild-type activity, residue involved in binding of pyridoxal 5'-phosphate | Homo sapiens |
Y114F | the mutation leads to significant increase in the production of H2S by CSE | Homo sapiens |
Y60A | 13% of wild-type activity | Homo sapiens |
Y60A | the mutation results in a severe decrease of enzyme activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
L-cysteine | mutant enzyme E339Y, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
1.9 | - |
L-cysteine | wild type enzyme, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
3.3 | - |
L-cysteine | mutant enzyme E339K, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
7.7 | - |
L-cysteine | mutant enzyme E339A, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose bead chromatography, gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Homo sapiens | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cysteine + H2O | - |
Homo sapiens | sulfide + NH3 + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CSE | - |
Homo sapiens |
cystathionine-gamma-lyase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-cysteine | wild type enzyme, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
0.34 | - |
L-cysteine | mutant enzyme E339K, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
0.76 | - |
L-cysteine | mutant enzyme E339Y, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
1.44 | - |
L-cysteine | mutant enzyme E339A, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens | |
pyridoxal 5'-phosphate | residues K212 and Y114 are involved in binding | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
L-cysteine | wild type enzyme, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
0.11 | - |
L-cysteine | mutant enzyme E339K, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
0.19 | - |
L-cysteine | mutant enzyme E339A, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens | |
0.43 | - |
L-cysteine | mutant enzyme E339Y, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C | Homo sapiens |