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Literature summary for 4.4.1.1 extracted from

  • Huang, S.; Chua, J.H.; Yew, W.S.; Sivaraman, J.; Moore, P.K.; Tan, C.H.; Deng, L.W.
    Site-directed mutagenesis on human cystathionine-gamma-lyase reveals insights into the modulation of H2S production (2009), J. Mol. Biol., 396, 708-718.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D187A 0.5% of wild-type activity Homo sapiens
D187A the mutation results in a loss of enzyme activity Homo sapiens
E339A the mutant demonstrates an approximately 6fold enhancement in H2S production Homo sapiens
E339A about 6fold enhancement in H2S production Homo sapiens
E339K the mutant shows increased catalytic efficiency compared to the wild type enzyme Homo sapiens
E339Y the mutant shows increased catalytic efficiency compared to the wild type enzyme Homo sapiens
E349A activity similar to wild-type Homo sapiens
E349A the mutant displays enzyme activity comparable to that for wild type enzyme Homo sapiens
K212A the mutation results in a loss of enzyme activity Homo sapiens
K212A 1.3% of wild-type activity, residue involved in binding of pyridoxal 5'-phosphate Homo sapiens
R375A 2% of wild-type activity Homo sapiens
R375A the mutation results in a loss of enzyme activity Homo sapiens
R62A 3% of wild-type activity Homo sapiens
R62A the mutation results in a loss of enzyme activity Homo sapiens
S209A activity similar to wild-type Homo sapiens
S209A the mutant displays enzyme activity comparable to that for wild type enzyme Homo sapiens
T189A 0.5% of wild-type activity Homo sapiens
T189A the mutation results in a loss of enzyme activity Homo sapiens
T211A activity similar to wild-type Homo sapiens
T211A the mutant displays enzyme activity comparable to that for wild type enzyme Homo sapiens
Y114A the mutation results in a loss of enzyme activity Homo sapiens
Y114A 3% of wild-type activity, residue involved in binding of pyridoxal 5'-phosphate Homo sapiens
Y114F the mutation leads to significant increase in the production of H2S by CSE Homo sapiens
Y60A 13% of wild-type activity Homo sapiens
Y60A the mutation results in a severe decrease of enzyme activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
L-cysteine mutant enzyme E339Y, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
1.9
-
L-cysteine wild type enzyme, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
3.3
-
L-cysteine mutant enzyme E339K, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
7.7
-
L-cysteine mutant enzyme E339A, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose bead chromatography, gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
Homo sapiens L-cysteine + 2-oxobutanoate + NH3
-
?
L-cysteine + H2O
-
Homo sapiens sulfide + NH3 + pyruvate
-
?

Synonyms

Synonyms Comment Organism
CSE
-
Homo sapiens
cystathionine-gamma-lyase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
L-cysteine wild type enzyme, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
0.34
-
L-cysteine mutant enzyme E339K, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
0.76
-
L-cysteine mutant enzyme E339Y, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
1.44
-
L-cysteine mutant enzyme E339A, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens
pyridoxal 5'-phosphate residues K212 and Y114 are involved in binding Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.06
-
L-cysteine wild type enzyme, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
0.11
-
L-cysteine mutant enzyme E339K, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
0.19
-
L-cysteine mutant enzyme E339A, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens
0.43
-
L-cysteine mutant enzyme E339Y, in 50 mM sodium phosphate buffer (pH 8.2) , at 37°C Homo sapiens