Cloned (Comment) | Organism |
---|---|
the His-tagged enzyme is expressed in Escherichia coli KS1000 cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E333A | the mutation leads to 30fold reduction of kcat/Km for L-cystathionine | Saccharomyces cerevisiae |
E333Y | the mutation leads to 30fold reduction of kcat/Km for L-cystathionine | Saccharomyces cerevisiae |
E48A | the mutation leads to about 6fold reduction of kcat/Km for L-cystathionine | Saccharomyces cerevisiae |
E48D | the mutation leads to about 5fold reduction of kcat/Km for L-cystathionine | Saccharomyces cerevisiae |
E48F | the mutation leads to about 9fold reduction of kcat/Km for L-cystathionine | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
L-cystathionine | His-tagged recombinant wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.45 | - |
L-cystathionine | native wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.81 | - |
L-cystathionine | mutant enzyme E48F, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.82 | - |
L-cystathionine | mutant enzyme E48A, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
1.11 | - |
L-cystathionine | mutant enzyme E48D, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
5.2 | - |
L-cystathionine | mutant enzyme E333A, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, butyl-Toyopearl 650M resin column chromatography, and DEAE column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Saccharomyces cerevisiae | L-homocysteine + pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cgl | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
L-cystathionine | mutant enzyme E48F, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.67 | - |
L-cystathionine | mutant enzyme E48A, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.69 | - |
L-cystathionine | mutant enzyme E333A, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.76 | - |
L-cystathionine | mutant enzyme E48D, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.9 | - |
L-cystathionine | native wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
1.81 | - |
L-cystathionine | His-tagged recombinant wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.132 | - |
L-cystathionine | mutant enzyme E333A, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.147 | - |
L-cystathionine | mutant enzyme E333Y, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.49 | - |
L-cystathionine | mutant enzyme E48F, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.68 | - |
L-cystathionine | mutant enzyme E48D, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
0.82 | - |
L-cystathionine | mutant enzyme E48A, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
1.98 | - |
L-cystathionine | native wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae | |
4.3 | - |
L-cystathionine | His-tagged recombinant wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C | Saccharomyces cerevisiae |