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Literature summary for 4.4.1.1 extracted from

  • Farsi, A.; Lodha, P.H.; Skanes, J.E.; Los, H.; Kalidindi, N.; Aitken, S.M.
    Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and development of tools for the investigation of their mechanisms (2009), Biochem. Cell Biol., 87, 445-457.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the His-tagged enzyme is expressed in Escherichia coli KS1000 cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
E333A the mutation leads to 30fold reduction of kcat/Km for L-cystathionine Saccharomyces cerevisiae
E333Y the mutation leads to 30fold reduction of kcat/Km for L-cystathionine Saccharomyces cerevisiae
E48A the mutation leads to about 6fold reduction of kcat/Km for L-cystathionine Saccharomyces cerevisiae
E48D the mutation leads to about 5fold reduction of kcat/Km for L-cystathionine Saccharomyces cerevisiae
E48F the mutation leads to about 9fold reduction of kcat/Km for L-cystathionine Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
L-cystathionine His-tagged recombinant wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.45
-
L-cystathionine native wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.81
-
L-cystathionine mutant enzyme E48F, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.82
-
L-cystathionine mutant enzyme E48A, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
1.11
-
L-cystathionine mutant enzyme E48D, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
5.2
-
L-cystathionine mutant enzyme E333A, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, butyl-Toyopearl 650M resin column chromatography, and DEAE column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
Saccharomyces cerevisiae L-homocysteine + pyruvate + NH3
-
?

Synonyms

Synonyms Comment Organism
cgl
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
L-cystathionine mutant enzyme E48F, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.67
-
L-cystathionine mutant enzyme E48A, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.69
-
L-cystathionine mutant enzyme E333A, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.76
-
L-cystathionine mutant enzyme E48D, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.9
-
L-cystathionine native wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
1.81
-
L-cystathionine His-tagged recombinant wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.132
-
L-cystathionine mutant enzyme E333A, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.147
-
L-cystathionine mutant enzyme E333Y, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.49
-
L-cystathionine mutant enzyme E48F, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.68
-
L-cystathionine mutant enzyme E48D, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
0.82
-
L-cystathionine mutant enzyme E48A, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
1.98
-
L-cystathionine native wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae
4.3
-
L-cystathionine His-tagged recombinant wild type enzyme, in 50 mM potassium phosphate, pH 7.8, at 25°C Saccharomyces cerevisiae