Cloned (Comment) | Organism |
---|---|
site directed mutagenesis mutants, expression in Escherichia coli | Treponema denticola |
Protein Variants | Comment | Organism |
---|---|---|
K238A | mutant with complete loss of lyase and racemase activity | Treponema denticola |
Y123F | mutant with decreased activity | Treponema denticola |
Y123F/Y124F | mutant with decreased activity | Treponema denticola |
Y124F | mutant with 50% of kcat for transamination of L- and D-alanine | Treponema denticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
beta-chloro-L-alanine | Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
0.17 | - |
beta-chloro-L-alanine | Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
0.76 | - |
D-alanine | racemization, Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
1 | - |
L-alanine | racemization, Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
1.21 | - |
beta-chloro-L-alanine | wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
3.2 | - |
beta-chloro-L-alanine | Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
10 | - |
L-alanine | racemization, wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
10 | - |
D-alanine | racemization, wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
14.3 | - |
L-alanine | racemization, Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
20 | - |
L-alanine | racemization, Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
22.4 | - |
D-alanine | racemization, Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola | |
25.3 | - |
D-alanine | racemization, Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C | Treponema denticola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chloro-L-alanine + H2O | - |
Treponema denticola | pyruvate + NH3 + HCl | - |
? | |
D-alanine + H2O | - |
Treponema denticola | ? | - |
? | |
L-alanine + H2O | - |
Treponema denticola | ? | - |
? | |
L-cysteine + H2O | - |
Treponema denticola | sulfide + NH3 + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Treponema denticola |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | enzyme binds 2 mol per dimer | Treponema denticola |