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Literature summary for 4.4.1.1 extracted from

  • Cellini, B.; Bertoldi, M.; Paiardini, A.; D'Aguanno, S.; Voltattorni, C.B.
    Site-directed mutagenesis provides insight into racemization and transamination of alanine catalyzed by Treponema denticola cystalysin (2004), J. Biol. Chem., 279, 36898-36905.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
site directed mutagenesis mutants, expression in Escherichia coli Treponema denticola

Protein Variants

Protein Variants Comment Organism
K238A mutant with complete loss of lyase and racemase activity Treponema denticola
Y123F mutant with decreased activity Treponema denticola
Y123F/Y124F mutant with decreased activity Treponema denticola
Y124F mutant with 50% of kcat for transamination of L- and D-alanine Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
beta-chloro-L-alanine Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
0.17
-
beta-chloro-L-alanine Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
0.76
-
D-alanine racemization, Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
1
-
L-alanine racemization, Y123F/Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
1.21
-
beta-chloro-L-alanine wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
3.2
-
beta-chloro-L-alanine Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
10
-
L-alanine racemization, wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
10
-
D-alanine racemization, wild type, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
14.3
-
L-alanine racemization, Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
20
-
L-alanine racemization, Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
22.4
-
D-alanine racemization, Y123F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola
25.3
-
D-alanine racemization, Y124F mutant, 20 mM potassium phosphate buffer, pH 7.4, 25°C Treponema denticola

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloro-L-alanine + H2O
-
Treponema denticola pyruvate + NH3 + HCl
-
?
D-alanine + H2O
-
Treponema denticola ?
-
?
L-alanine + H2O
-
Treponema denticola ?
-
?
L-cysteine + H2O
-
Treponema denticola sulfide + NH3 + pyruvate
-
?

Subunits

Subunits Comment Organism
homodimer
-
Treponema denticola

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate enzyme binds 2 mol per dimer Treponema denticola