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Literature summary for 4.4.1.1 extracted from

  • Yoshida, Y.; Nakano, Y.; Amano, A.; Yoshimura, M.; Fukamachi, H.; Oho, T.; Koga, T.
    lcd from Streptococcus anginosus encodes a C-S lyase with alpha,beta-elimination activity that degrades L-cysteine (2002), Microbiology, 148, 3961-3970.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Streptococcus anginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
L-cysteine pH 8.0, 37°C Streptococcus anginosus
1.29
-
L-cystine pH 8.0, 37°C Streptococcus anginosus
2.75
-
S-(2-aminoethyl)-L-cysteine pH 8.0, 37°C Streptococcus anginosus
3.27
-
DL-cystathionine pH 8.0, 37°C Streptococcus anginosus
13.13
-
S-methyl-L-cysteine pH 8.0, 37°C Streptococcus anginosus
28.41
-
3-chloro-DL-alanine pH 8.0, 37°C Streptococcus anginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Streptococcus anginosus

Organism

Organism UniProt Comment Textmining
Streptococcus anginosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus anginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-DL-alanine + H2O
-
Streptococcus anginosus NH3 + ?
-
?
DL-cystathionine + H2O
-
Streptococcus anginosus L-cysteine + NH3 + 2-oxobutanoate
-
?
L-cysteine + H2O
-
Streptococcus anginosus sulfide + NH3 + pyruvate
-
?
L-cystine + 2 H2O
-
Streptococcus anginosus 2 NH3 + 2 pyruvate + 2 hydrogen sulfide
-
?
L-methionine + H2O
-
Streptococcus anginosus NH3 + ?
-
?
S-(2-aminoethyl)-L-cysteine + H2O
-
Streptococcus anginosus NH3 + pyruvate + cysteamine
-
?
S-methyl-L-cysteine + H2O
-
Streptococcus anginosus NH3 + pyruvate + methyl mercaptan
-
?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Streptococcus anginosus