Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.1 extracted from

  • De Angelis, M.; Curtin, A.C.; McSweeney, P.L.; Faccia, M.; Gobbetti, M.
    Lactobacillus reuteri DSM 20016: purification and characterization of a cystathionine gamma-lyase and use as adjunct starter in cheesemaking (2002), J. Dairy Res., 69, 255-267.
    View publication on PubMed

Application

Application Comment Organism
biotechnology use of strain as adjunct starter in cheese making Limosilactobacillus reuteri

Inhibitors

Inhibitors Comment Organism Structure
3-methyl-2-benzothiazolinone 31% residual activity at 5 mM Limosilactobacillus reuteri
DL-Penicillamine 3% residual activity at 5 mM Limosilactobacillus reuteri
DL-propargylglycine 2% residual activity at 5 mM Limosilactobacillus reuteri
iodoacetamide 22% residual activity at 2.5 mM Limosilactobacillus reuteri
additional information not inhibitory: EDTA Limosilactobacillus reuteri
N-ethylmaleimide 81% residual activity at 5 mM Limosilactobacillus reuteri
NaCl 59% residual activity at 5 mM Limosilactobacillus reuteri
phenylhydrazine 2.8% residual activity at 5 mM Limosilactobacillus reuteri
Semicarbazide 11% residual activity at 5 mM Limosilactobacillus reuteri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Limosilactobacillus reuteri
160000
-
gel filtration Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri
-
DSM 20016
-

Purification (Commentary)

Purification (Comment) Organism
-
Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathione + H2O
-
Limosilactobacillus reuteri L-cysteine + NH3 + 2-oxobutanoate
-
?
L-cysteine + H2O
-
Limosilactobacillus reuteri sulfide + NH3 + pyruvate
-
?
L-cystine + H2O
-
Limosilactobacillus reuteri NH3 + ?
-
?
L-djenkolic acid + H2O
-
Limosilactobacillus reuteri NH3 + ?
-
?
L-methionine + H2O
-
Limosilactobacillus reuteri NH3 + ?
-
?
L-serine + H2O
-
Limosilactobacillus reuteri NH3 + ?
-
?
additional information activity decreases in the following order of substrates: L-cystathione, L-djenkolic, L-cystine, L-cysteine, L-methionine, L-serine Limosilactobacillus reuteri ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Limosilactobacillus reuteri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15
-
5 min, enzyme maintains about 50% of activity Limosilactobacillus reuteri
60
-
5 min, complete inactivation Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Limosilactobacillus reuteri

pH Range

pH Minimum pH Maximum Comment Organism
5
-
20% residual activity Limosilactobacillus reuteri
6
-
40% residual activity Limosilactobacillus reuteri
9
-
90% residual activity Limosilactobacillus reuteri

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Limosilactobacillus reuteri

pI Value

Organism Comment pI Value Maximum pI Value
Limosilactobacillus reuteri isoelectric focusing
-
5