Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces phaeochromogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
beta-cyano-L-Ala | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
cycloserine | - |
Streptomyces phaeochromogenes | |
Hg2+ | HgCl2, alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
hydroxylamine | alpha,beta-elimination of L-homoserine | Streptomyces phaeochromogenes | |
PCMB | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
Penicillamine | alpha,beta-elimination of L-homoserine; D-penicillamine; L-penicillamine | Streptomyces phaeochromogenes | |
phenylhydrazine | alpha,beta-elimination L-homoserine as substrate | Streptomyces phaeochromogenes | |
propargylglycine | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
Semicarbazide | alpha,beta-elimination L-homoserine as substrate | Streptomyces phaeochromogenes | |
Sodium cyanide | alpha,beta-elimination, 1 mM, 82% inhibition | Streptomyces phaeochromogenes | |
Zn2+ | ZnCl2, alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.204 | - |
L-cystathionine | - |
Streptomyces phaeochromogenes | |
0.209 | - |
DL-lanthionine | - |
Streptomyces phaeochromogenes | |
0.909 | - |
L-cystine | - |
Streptomyces phaeochromogenes | |
2.39 | - |
beta-chloro-L-Ala | - |
Streptomyces phaeochromogenes | |
5.18 | - |
DL-vinylglycine | - |
Streptomyces phaeochromogenes | |
12.5 | - |
L-homoserine | - |
Streptomyces phaeochromogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, SDS-PAGE | Streptomyces phaeochromogenes |
162000 | - |
gel filtration | Streptomyces phaeochromogenes |
168000 | - |
equilibrium sedimentation | Streptomyces phaeochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis lactamdurans | - |
- |
- |
Gordonia rubripertincta | - |
- |
- |
Micromonospora chalcea | - |
- |
- |
Micromonospora coerulea | - |
- |
- |
Mycolicibacterium phlei | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Nocardia asteroides | - |
- |
- |
Nocardia erythropolis | - |
- |
- |
Nocardia globerula | - |
- |
- |
Nonomuraea angiospora | - |
- |
- |
Streptomyces lydicus | - |
- |
- |
Streptomyces microflavus | - |
- |
- |
Streptomyces netropsis | - |
- |
- |
Streptomyces olivochromogenes | - |
- |
- |
Streptomyces phaeochromogenes | - |
IFO 3105 | - |
Streptomyces rimosus | - |
- |
- |
Streptomyces virginiae | - |
- |
- |
Streptosporangium roseum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces phaeochromogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
- |
Streptomyces phaeochromogenes |
Storage Stability | Organism |
---|---|
-20°C, in 45% glycerol solution, containing 10 mM potassium phosphate, pH 7.5, 0.02 mM pyridoxal 5'-phosphate, 0.12 mM EDTA, 0.2 mM DTT, stable for 2 months or more | Streptomyces phaeochromogenes |
4°C, 10 mM potassium phosphate, pH 7.5, containing 0.02 mM pyridoxal phosphate, 0.12 mM EDTA, 0.2 mM dithiothreitol stable for 2 weeks | Streptomyces phaeochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chloro-L-Ala | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
beta-chloro-L-Ala | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
djenkolic acid | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
djenkolic acid | alpha-gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
DL-vinylglycine | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
DL-vinylglycine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
L-cystathionine + H2O | alpha,gamma-elimination | Streptomyces phaeochromogenes | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cystathionine + H2O | alpha,beta-elimination | Streptomyces phaeochromogenes | L-homocysteine + pyruvate + NH3 | - |
? | |
L-cystine | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
L-cystine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
L-homoserine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
Lanthionine | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
Lanthionine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
Lanthionine | DL-lanthionine | Streptomyces phaeochromogenes | ? | - |
? | |
S-methyl-L-Cys | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Streptomyces phaeochromogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces phaeochromogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
0.04 mM pyridoxal 5'-phosphate, 10 min, stable | Streptomyces phaeochromogenes |
55 | - |
0.04 mM pyridoxal 5'-phosphate, 10 min, 15% loss of activity | Streptomyces phaeochromogenes |
60 | - |
0.04 mM pyridoxal 5'-phosphate, 10 min, 50% loss of activity | Streptomyces phaeochromogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces phaeochromogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme | Streptomyces phaeochromogenes |