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Literature summary for 4.4.1.1 extracted from

  • Nagasawa, T.; Kanzaki, H.; Yamada, H.
    Cystathionine gamma-lyase of Streptomyces phaeochromogenes. The occurrence of cystathionine gamma-lyase in filamentous bacteria and its purification and characterization (1984), J. Biol. Chem., 259, 10393-10403.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Streptomyces phaeochromogenes

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
beta-cyano-L-Ala alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
cycloserine
-
Streptomyces phaeochromogenes
Hg2+ HgCl2, alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
hydroxylamine alpha,beta-elimination of L-homoserine Streptomyces phaeochromogenes
PCMB alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
Penicillamine alpha,beta-elimination of L-homoserine; D-penicillamine; L-penicillamine Streptomyces phaeochromogenes
phenylhydrazine alpha,beta-elimination L-homoserine as substrate Streptomyces phaeochromogenes
propargylglycine alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
Semicarbazide alpha,beta-elimination L-homoserine as substrate Streptomyces phaeochromogenes
Sodium cyanide alpha,beta-elimination, 1 mM, 82% inhibition Streptomyces phaeochromogenes
Zn2+ ZnCl2, alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.204
-
L-cystathionine
-
Streptomyces phaeochromogenes
0.209
-
DL-lanthionine
-
Streptomyces phaeochromogenes
0.909
-
L-cystine
-
Streptomyces phaeochromogenes
2.39
-
beta-chloro-L-Ala
-
Streptomyces phaeochromogenes
5.18
-
DL-vinylglycine
-
Streptomyces phaeochromogenes
12.5
-
L-homoserine
-
Streptomyces phaeochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
4 * 41000, SDS-PAGE Streptomyces phaeochromogenes
162000
-
gel filtration Streptomyces phaeochromogenes
168000
-
equilibrium sedimentation Streptomyces phaeochromogenes

Organism

Organism UniProt Comment Textmining
Amycolatopsis lactamdurans
-
-
-
Gordonia rubripertincta
-
-
-
Micromonospora chalcea
-
-
-
Micromonospora coerulea
-
-
-
Mycolicibacterium phlei
-
-
-
Mycolicibacterium smegmatis
-
-
-
Nocardia asteroides
-
-
-
Nocardia erythropolis
-
-
-
Nocardia globerula
-
-
-
Nonomuraea angiospora
-
-
-
Streptomyces lydicus
-
-
-
Streptomyces microflavus
-
-
-
Streptomyces netropsis
-
-
-
Streptomyces olivochromogenes
-
-
-
Streptomyces phaeochromogenes
-
IFO 3105
-
Streptomyces rimosus
-
-
-
Streptomyces virginiae
-
-
-
Streptosporangium roseum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces phaeochromogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
-
Streptomyces phaeochromogenes

Storage Stability

Storage Stability Organism
-20°C, in 45% glycerol solution, containing 10 mM potassium phosphate, pH 7.5, 0.02 mM pyridoxal 5'-phosphate, 0.12 mM EDTA, 0.2 mM DTT, stable for 2 months or more Streptomyces phaeochromogenes
4°C, 10 mM potassium phosphate, pH 7.5, containing 0.02 mM pyridoxal phosphate, 0.12 mM EDTA, 0.2 mM dithiothreitol stable for 2 weeks Streptomyces phaeochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloro-L-Ala alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
beta-chloro-L-Ala alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
djenkolic acid alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
djenkolic acid alpha-gamma-elimination Streptomyces phaeochromogenes ?
-
?
DL-vinylglycine alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
DL-vinylglycine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
L-cystathionine + H2O alpha,gamma-elimination Streptomyces phaeochromogenes L-cysteine + 2-oxobutanoate + NH3
-
?
L-cystathionine + H2O alpha,beta-elimination Streptomyces phaeochromogenes L-homocysteine + pyruvate + NH3
-
?
L-cystine alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
L-cystine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
L-homoserine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
Lanthionine alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
Lanthionine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
Lanthionine DL-lanthionine Streptomyces phaeochromogenes ?
-
?
S-methyl-L-Cys alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Streptomyces phaeochromogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Streptomyces phaeochromogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
0.04 mM pyridoxal 5'-phosphate, 10 min, stable Streptomyces phaeochromogenes
55
-
0.04 mM pyridoxal 5'-phosphate, 10 min, 15% loss of activity Streptomyces phaeochromogenes
60
-
0.04 mM pyridoxal 5'-phosphate, 10 min, 50% loss of activity Streptomyces phaeochromogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Streptomyces phaeochromogenes

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Streptomyces phaeochromogenes