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Literature summary for 4.4.1.1 extracted from

  • Chu, L.; Ebersole, J.L.; Kurzban, G.P.; Holt, S.C.
    Cystalysin, a 46-kDa L-cysteine desulfhydrase from Treponema denticola: biochemical and biophysical characterization (1999), Clin. Infect. Dis., 28, 442-450.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol increases activity Treponema denticola

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Treponema denticola

Inhibitors

Inhibitors Comment Organism Structure
Ala competitive Treponema denticola
Gly competitive Treponema denticola
L-Asn weak Treponema denticola
L-Met weak Treponema denticola
L-Thr weak Treponema denticola
L-Val weak Treponema denticola
Ser competitive Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4
-
Cys
-
Treponema denticola

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
LC-67
-
Treponema denticola LC-67
-
LC-67
-

Purification (Commentary)

Purification (Comment) Organism
-
Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Cys
-
Treponema denticola H2S + NH3 + pyruvate
-
?
L-Cys
-
Treponema denticola LC-67 H2S + NH3 + pyruvate
-
?
L-Cys
-
Treponema denticola ATCC 35404 H2S + NH3 + pyruvate
-
?
L-cystathionine + H2O
-
Treponema denticola L-homocysteine + pyruvate + NH3
-
?
L-cystathionine + H2O
-
Treponema denticola LC-67 L-homocysteine + pyruvate + NH3
-
?
L-cystathionine + H2O
-
Treponema denticola ATCC 35404 L-homocysteine + pyruvate + NH3
-
?
S-aminoethyl-L-Cys
-
Treponema denticola ?
-
?
S-aminoethyl-L-Cys
-
Treponema denticola LC-67 ?
-
?
S-aminoethyl-L-Cys
-
Treponema denticola ATCC 35404 ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
30 min, complete inactivation Treponema denticola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
Cys
-
Treponema denticola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8
-
Treponema denticola

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Treponema denticola