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Literature summary for 4.3.99.4 extracted from

  • Craciun, S.; Balskus, E.P.
    Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme (2012), Proc. Natl. Acad. Sci. USA, 109, 21307-21312.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.99.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oleidesulfovibrio alaskensis

Protein Variants

Protein Variants Comment Organism
C489A complete loss of activity Oleidesulfovibrio alaskensis
G821A complete loss of activity Oleidesulfovibrio alaskensis

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
-
-
Desulfovibrio desulfuricans B8J0I2 member of the glycyl radical enzyme family
-
Oleidesulfovibrio alaskensis Q30W70 member of the glycyl radical enzyme family
-
Oleidesulfovibrio alaskensis G20 Q30W70 member of the glycyl radical enzyme family
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline
-
 Oleidesulfovibrio alaskensis trimethylamine + acetaldehyde a glycyl radical-based mechanism of C-N cleavage ?
choline
-
 Desulfovibrio desulfuricans trimethylamine + acetaldehyde a glycyl radical-based mechanism of C-N cleavage ?
choline
-
 Oleidesulfovibrio alaskensis G20 trimethylamine + acetaldehyde a glycyl radical-based mechanism of C-N cleavage ?

Synonyms

Synonyms Comment Organism
choline TMA-lyase
-
 Oleidesulfovibrio alaskensis
choline TMA-lyase
-
 Desulfovibrio desulfuricans
cutC
-
 Oleidesulfovibrio alaskensis
cutC
-
 Desulfovibrio desulfuricans
Dde_3282 locus name Oleidesulfovibrio alaskensis
Dde_3282 locus name Desulfovibrio desulfuricans

General Information

General Information Comment Organism
physiological function gene disruption impairs growth on choline and abolishes production of trimethylamine, with no difference in growth between wild-type and mutant in media containing lactate. Expression of choline trimethylamine-lyase CutC and its activating protein CutD in Escherichia coli BL21 results in production of d9-TMA from (trimethyl-d9)-choline. Expression of either enzyme on its own completely abolishes trimethylamine production Oleidesulfovibrio alaskensis