Application | Comment | Organism |
---|---|---|
drug development | the enzyme is an anti-cholera target | Vibrio cholerae |
pharmacology | the enzyme is an anti-cholera target | Vibrio cholerae |
Cloned (Comment) | Organism |
---|---|
- |
Vibrio cholerae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-aspartate-4-semialdehyde | pH 8.0, 30°C, untagged enzyme | Vibrio cholerae | |
0.09 | - |
L-aspartate-4-semialdehyde | pH 8.0, 30°C, His-tagged enzyme | Vibrio cholerae | |
0.14 | - |
pyruvate | pH 8.0, 30°C, untagged enzyme | Vibrio cholerae | |
0.15 | - |
pyruvate | pH 8.0, 30°C, His-tagged enzyme | Vibrio cholerae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
recombinant untagged recombinant enzyme | Vibrio cholerae |
68000 | - |
recombinant His-tagged recombinant enzyme | Vibrio cholerae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + L-aspartate-4-semialdehyde | Vibrio cholerae | the enzyme catalyzes the first committed step in the diaminopimelate pathway | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? | |
pyruvate + L-aspartate-4-semialdehyde | Vibrio cholerae O395 | the enzyme catalyzes the first committed step in the diaminopimelate pathway | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae | A5F699 | - |
- |
Vibrio cholerae O395 | A5F699 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant untagged and His-tagged enzymes | Vibrio cholerae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
75.6 | - |
pH 8.0, 30°C, untagged enzyme | Vibrio cholerae |
112 | - |
pH 8.0, 30°C, His-tagged enzyme | Vibrio cholerae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + L-aspartate-4-semialdehyde | - |
Vibrio cholerae | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? | |
pyruvate + L-aspartate-4-semialdehyde | the enzyme catalyzes the first committed step in the diaminopimelate pathway | Vibrio cholerae | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? | |
pyruvate + L-aspartate-4-semialdehyde | - |
Vibrio cholerae O395 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? | |
pyruvate + L-aspartate-4-semialdehyde | the enzyme catalyzes the first committed step in the diaminopimelate pathway | Vibrio cholerae O395 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31000, SDS-PAGE, recombinant untagged recombinant enzyme | Vibrio cholerae |
dimer | 2 * 33000, SDS-PAGE, recombinant His-tagged recombinant enzyme | Vibrio cholerae |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Vibrio cholerae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
pyruvate | pH 8.0, 30°C, untagged enzyme | Vibrio cholerae | |
34 | - |
L-aspartate-4-semialdehyde | pH 8.0, 30°C, untagged enzyme | Vibrio cholerae | |
46 | - |
pyruvate | pH 8.0, 30°C, His-tagged enzyme | Vibrio cholerae | |
46 | - |
L-aspartate-4-semialdehyde | pH 8.0, 30°C, His-tagged enzyme | Vibrio cholerae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first committed step in the diaminopimelate pathway | Vibrio cholerae |