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Literature summary for 4.3.3.7 extracted from

  • Gupta, R.; Soares da Costa, T.P.; Faou, P.; Dogovski, C.; Perugini, M.A.
    Comparison of untagged and his-tagged dihydrodipicolinate synthase from the enteric pathogen Vibrio cholerae (2018), Protein Expr. Purif., 145, 85-93 .
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme is an anti-cholera target Vibrio cholerae
pharmacology the enzyme is an anti-cholera target Vibrio cholerae

Cloned(Commentary)

Cloned (Comment) Organism
-
Vibrio cholerae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
L-aspartate-4-semialdehyde pH 8.0, 30°C, untagged enzyme Vibrio cholerae
0.09
-
L-aspartate-4-semialdehyde pH 8.0, 30°C, His-tagged enzyme Vibrio cholerae
0.14
-
pyruvate pH 8.0, 30°C, untagged enzyme Vibrio cholerae
0.15
-
pyruvate pH 8.0, 30°C, His-tagged enzyme Vibrio cholerae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
recombinant untagged recombinant enzyme Vibrio cholerae
68000
-
recombinant His-tagged recombinant enzyme Vibrio cholerae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + L-aspartate-4-semialdehyde Vibrio cholerae the enzyme catalyzes the first committed step in the diaminopimelate pathway (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
?
pyruvate + L-aspartate-4-semialdehyde Vibrio cholerae O395 the enzyme catalyzes the first committed step in the diaminopimelate pathway (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae A5F699
-
-
Vibrio cholerae O395 A5F699
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant untagged and His-tagged enzymes Vibrio cholerae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75.6
-
pH 8.0, 30°C, untagged enzyme Vibrio cholerae
112
-
pH 8.0, 30°C, His-tagged enzyme Vibrio cholerae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + L-aspartate-4-semialdehyde
-
Vibrio cholerae (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
?
pyruvate + L-aspartate-4-semialdehyde the enzyme catalyzes the first committed step in the diaminopimelate pathway Vibrio cholerae (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
?
pyruvate + L-aspartate-4-semialdehyde
-
Vibrio cholerae O395 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
?
pyruvate + L-aspartate-4-semialdehyde the enzyme catalyzes the first committed step in the diaminopimelate pathway Vibrio cholerae O395 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 31000, SDS-PAGE, recombinant untagged recombinant enzyme Vibrio cholerae
dimer 2 * 33000, SDS-PAGE, recombinant His-tagged recombinant enzyme Vibrio cholerae

Synonyms

Synonyms Comment Organism
DHDPS
-
Vibrio cholerae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
34
-
pyruvate pH 8.0, 30°C, untagged enzyme Vibrio cholerae
34
-
L-aspartate-4-semialdehyde pH 8.0, 30°C, untagged enzyme Vibrio cholerae
46
-
pyruvate pH 8.0, 30°C, His-tagged enzyme Vibrio cholerae
46
-
L-aspartate-4-semialdehyde pH 8.0, 30°C, His-tagged enzyme Vibrio cholerae

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first committed step in the diaminopimelate pathway Vibrio cholerae