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Literature summary for 4.3.3.7 extracted from

  • Atkinson, S.; Hor, L.; Dogovski, C.; Dobson, R.; Perugini, M.
    Identification of the bona fide DHDPS from a common plant pathogen (2014), Proteins, 82, 1869-1883.
    View publication on PubMed
Search for more literature for 4.3.3.7

Application

Application Comment Organism
drug development the enzyme is a bona fide drug target for treatment of the Crown Gall disease on crops caused by Agrobacterium tumefaciens, rational design of pesticide agents Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment) Organism
gene dapA1, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA10, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA2, sequence comparisons, expression of the His-tagged isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA3, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA4, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA5, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA6, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA7, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA8, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens
gene dapA9, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis, PDB ID 2HMC, structure comparisons Agrobacterium tumefaciens
crystal structure analysis, PDB ID 2R8Wm, structure comparisons Agrobacterium tumefaciens
crystal structure analysis, PDB ID 3B4U, structure comparisons Agrobacterium tumefaciens
crystal structure analysis, PDB IDs 4I7U, 4I7V, and 4I7W, resolution at 1.42-1.69 A, structure comparisons Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
(S)-aspartate-4-semialdehyde substrate inhibition Agrobacterium tumefaciens
(S)-lysine allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling Agrobacterium tumefaciens
pyruvate substrate inhibition Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16 0.32 (S)-aspartate-4-semialdehyde pH and temperature not specified in the publication Agrobacterium tumefaciens
0.31 0.44 pyruvate pH and temperature not specified in the publication Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate Agrobacterium tumefaciens
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
(S)-aspartate-4-semialdehyde + pyruvate Agrobacterium tumefaciens C58 / ATCC 33970
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A9CFV4 dapA10; gene dapA10
-
Agrobacterium tumefaciens A9CGZ4 dapA8; gene dapA8
-
Agrobacterium tumefaciens A9CHR2 dapA5; gene dapA5
-
Agrobacterium tumefaciens A9CL94 dapA2; genes dapA2
-
Agrobacterium tumefaciens A9CL97 dapA3; gene dapA3
-
Agrobacterium tumefaciens Q7CU96 dapA9; gene dapA9
-
Agrobacterium tumefaciens Q7D0E8 dapA6; gene dapA6
-
Agrobacterium tumefaciens Q7D313 dapA1; gene dapA1
-
Agrobacterium tumefaciens Q7D3Z9 dapA4; gene dapA4
-
Agrobacterium tumefaciens Q8UGL3 dapA7; gene dapA7
-
Agrobacterium tumefaciens C58 / ATCC 33970 A9CFV4 dapA10; gene dapA10
-
Agrobacterium tumefaciens C58 / ATCC 33970 A9CGZ4 dapA8; gene dapA8
-
Agrobacterium tumefaciens C58 / ATCC 33970 A9CHR2 dapA5; gene dapA5
-
Agrobacterium tumefaciens C58 / ATCC 33970 A9CL94 dapA2; genes dapA2
-
Agrobacterium tumefaciens C58 / ATCC 33970 A9CL97 dapA3; gene dapA3
-
Agrobacterium tumefaciens C58 / ATCC 33970 Q7CU96 dapA9; gene dapA9
-
Agrobacterium tumefaciens C58 / ATCC 33970 Q7D0E8 dapA6; gene dapA6
-
Agrobacterium tumefaciens C58 / ATCC 33970 Q7D313 dapA1; gene dapA1
-
Agrobacterium tumefaciens C58 / ATCC 33970 Q7D3Z9 dapA4; gene dapA4
-
Agrobacterium tumefaciens C58 / ATCC 33970 Q8UGL3 dapA7; gene dapA7
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate
-
 Agrobacterium tumefaciens (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
(S)-aspartate-4-semialdehyde + pyruvate bi-bi ping-pong substrate model Agrobacterium tumefaciens (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
(S)-aspartate-4-semialdehyde + pyruvate
-
 Agrobacterium tumefaciens C58 / ATCC 33970 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?
(S)-aspartate-4-semialdehyde + pyruvate bi-bi ping-pong substrate model Agrobacterium tumefaciens C58 / ATCC 33970 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer the enzyme adopts the canonical homotetrameric structure in both solution and the crystal state Agrobacterium tumefaciens

Synonyms

Synonyms Comment Organism
DHDPS
-
 Agrobacterium tumefaciens
dihydrodipicolinate synthase
-
 Agrobacterium tumefaciens
HTPA synthase UniProt Agrobacterium tumefaciens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.15
-
 (S)-aspartate-4-semialdehyde pH and temperature not specified in the publication Agrobacterium tumefaciens
0.19 0.57 pyruvate pH and temperature not specified in the publication Agrobacterium tumefaciens

General Information

General Information Comment Organism
metabolism the enzyme catalyses the first committed step in the lysine biosynthesis pathway, the condensation of pyruvate and (S)-aspartate semialdehyde to form (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid Agrobacterium tumefaciens
additional information the catalytic triad, consisting of Tyr133, Thr44, and Tyr107, acts as a proton relay to transfer protons to and from the active site via a water-filled channel leading to bulk solvent Agrobacterium tumefaciens