Crystallization (Comment) | Organism |
---|---|
purified recombinant DHDPS mutant A204R, sitting drop vapour diffusion method at room temperature, 200 nl of 10 mg/ml protein in 20 mM Tris-HCl, 2 mM 2-mercaptoethanol, 250 mM NaCl, 5% v/v glycerol, 10 mM pyruvate, pH 8.0, is mixed with 230 nl reservoir solution containing 2.0 M ammonium sulphate, 100 mM sodium acetate, pH 5.5, X-ray diffraction structure determination and analysis at 2.0 A resolution | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
A204R | site-directed mutagenesis, disruption of the native tetramer formation by targeting the dimer-dimer interface. The mutant enzyme is a dimeric protein with an identical fold and active-site structure to the tetrameric wild-type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
pyruvate | pH 8.0, 30°C, recombinant wild-type enzyme | Mycobacterium tuberculosis | |
0.33 | - |
pyruvate | pH 8.0, 30°C, recombinant mutant A204R | Mycobacterium tuberculosis | |
0.43 | - |
L-aspartate 4-semialdehyde | pH 8.0, 30°C, recombinant wild-type enzyme | Mycobacterium tuberculosis | |
1.1 | - |
L-aspartate 4-semialdehyde | pH 8.0, 30°C, recombinant mutant A204R | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62400 | - |
2 * 62400, mutant A204R, crystal structure | Mycobacterium tuberculosis |
62400 | - |
4 * 62400, crystal structure, the tetrameric structure is not essential for activity in DHDPS from Mycobacterium tuberculosis | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | Mycobacterium tuberculosis | - |
(S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pyruvate + L-aspartate-4-semialdehyde = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | reaction mechanism including Schiff base formation, tautomerization, and cyclization, overview | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | - |
Mycobacterium tuberculosis | (S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62400, mutant A204R, crystal structure | Mycobacterium tuberculosis |
tetramer | 4 * 62400, crystal structure, the tetrameric structure is not essential for activity in DHDPS from Mycobacterium tuberculosis | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
119 | - |
pyruvate | pH 8.0, 30°C, recombinant mutant A204R | Mycobacterium tuberculosis | |
119 | - |
L-aspartate 4-semialdehyde | pH 8.0, 30°C, recombinant mutant A204R | Mycobacterium tuberculosis | |
138 | - |
pyruvate | pH 8.0, 30°C, recombinant wild-type enzyme | Mycobacterium tuberculosis | |
138 | - |
L-aspartate 4-semialdehyde | pH 8.0, 30°C, recombinant wild-type enzyme | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.75 | 8.25 | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
additional information | the tetrameric structure is not essential for activity in DHDPS from Mycobacterium tuberculosis | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
L-aspartate 4-semialdehyde | pH 8.0, 30°C, recombinant mutant A204R | Mycobacterium tuberculosis | |
320 | - |
L-aspartate 4-semialdehyde | pH 8.0, 30°C, recombinant wild-type enzyme | Mycobacterium tuberculosis | |
360 | - |
pyruvate | pH 8.0, 30°C, recombinant mutant A204R | Mycobacterium tuberculosis | |
820 | - |
pyruvate | pH 8.0, 30°C, recombinant wild-type enzyme | Mycobacterium tuberculosis |