Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.3.7 extracted from

  • Wubben, J.M.; Dogovski, C.; Dobson, R.C.; Codd, R.; Gerrard, J.A.; Parker, M.W.; Perugini, M.A.
    Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica (2010), Acta Crystallogr. Sect. F, 66, 1511-1516.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dapA, expression of His-tagged DHDPS in Escherichia coli strain BL21(DE3) Shewanella benthica

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, hanging-drop vapour-diffusion method, 7.6 mg/ml protein in 150 nl solution is mixed with 150 nl of reservoir solution containing 200 mM ammonium sulfate, 100 mM Bis-Tris pH 5.0-6.0, 23-26% w/v PEG 3350, 0.02% w/v sodium azide, X-ray diffraction structure determination and analysis at 2.5 A resolution Shewanella benthica

Organism

Organism UniProt Comment Textmining
Shewanella benthica
-
gene dapA
-
Shewanella benthica ATCC 3392
-
gene dapA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged DHDPS 9.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Shewanella benthica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
104
-
purified recombinant His-tagged enzyme, pH not specified in the publication, temperature not specified in the publication Shewanella benthica

Synonyms

Synonyms Comment Organism
DHDPS
-
Shewanella benthica

General Information

General Information Comment Organism
metabolism DHDPS is an oligomeric enzyme that catalyzes the first committed step of the lysine biosynthesis pathway in plants and bacteria, which yields essential building blocks for cell-wall and protein synthesis Shewanella benthica