Cloned (Comment) | Organism |
---|---|
gene dapA, expression of His-tagged DHDPS in Escherichia coli strain BL21(DE3) | Shewanella benthica |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging-drop vapour-diffusion method, 7.6 mg/ml protein in 150 nl solution is mixed with 150 nl of reservoir solution containing 200 mM ammonium sulfate, 100 mM Bis-Tris pH 5.0-6.0, 23-26% w/v PEG 3350, 0.02% w/v sodium azide, X-ray diffraction structure determination and analysis at 2.5 A resolution | Shewanella benthica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella benthica | - |
gene dapA | - |
Shewanella benthica ATCC 3392 | - |
gene dapA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged DHDPS 9.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Shewanella benthica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
104 | - |
purified recombinant His-tagged enzyme, pH not specified in the publication, temperature not specified in the publication | Shewanella benthica |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Shewanella benthica |
General Information | Comment | Organism |
---|---|---|
metabolism | DHDPS is an oligomeric enzyme that catalyzes the first committed step of the lysine biosynthesis pathway in plants and bacteria, which yields essential building blocks for cell-wall and protein synthesis | Shewanella benthica |