Literature summary for 4.3.3.7 extracted from

Atkinson, S.C.; Dobson, R.C.; Newman, J.M.; Gorman, M.A.; Dogovski, C.; Parker, M.W.; Perugini, M.A.
Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate (2009), Acta Crystallogr. Sect. F, 65, 253-255.

Cloned(Commentary)

Cloned (Comment)	Organism
inserted into the vector pET11a and transformed into Escherichia coli strain CodonPlus BL21 (DE3)	Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment)	Organism
in the presence of its substrate pyruvate, by the sitting-drop vapour diffusion method, to 1.2 A resolution. Belongs to space group C2, in contrast to the unbound form, which has trigonal symmetry. Unit-cell parameters are a = 143.4, b = 54.8, c = 94.3 A , beta = 126.3. The crystal volume per protein weight is 2.3 A3 Da-1 (based on the presence of two monomers in the asymmetric unit), with an estimated solvent content of 46%	Clostridium botulinum

Crystallization (Comment)

Organism

in the presence of its substrate pyruvate, by the sitting-drop vapour diffusion method, to 1.2 A resolution. Belongs to space group C2, in contrast to the unbound form, which has trigonal symmetry. Unit-cell parameters are a = 143.4, b = 54.8, c = 94.3 A , beta = 126.3. The crystal volume per protein weight is 2.3 A3 Da-1 (based on the presence of two monomers in the asymmetric unit), with an estimated solvent content of 46%

Clostridium botulinum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31530	-	purified enzyme, mass spectrometry	Clostridium botulinum
31650	-	mass spectrometry	Clostridium botulinum

Organism

Organism	UniProt	Comment	Textmining
Clostridium botulinum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by anion-exchange chromatography, hydrophobic interaction and gel filtration	Clostridium botulinum

Storage Stability

Storage Stability	Organism
-20°C, 20 mM Tris-HCl buffer, pH 8.0	Clostridium botulinum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate 4-semialdehyde + pyruvate	-	Clostridium botulinum	dihydrodipicolinate + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
tetramer	crystallography	Clostridium botulinum

Synonyms

Synonyms	Comment	Organism
DapA	-	Clostridium botulinum
DHDPS	-	Clostridium botulinum
dihydrodipicolinate synthase	-	Clostridium botulinum