Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3), pET24d vector | Caldanaerobacter subterraneus subsp. tengcongensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-lysine | increasing amounts of (S)-lysine (0-200 mM) added, not inhibited under physiological conditions, binding site of the allosteric inhibitor lysine appears not to be conserved | Caldanaerobacter subterraneus subsp. tengcongensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
(S)-aspartate 4-semialdehyde | by adding 20 mM pyruvate, at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
0.85 | - |
pyruvate | by adding 2 mM (S)-aspartate 4-semialdehyde, at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
0.92 | - |
(S)-aspartate 4-semialdehyde | modeled with substrate inhibition, at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
size of monomer inccluding His-tag, calculated from sequence | Caldanaerobacter subterraneus subsp. tengcongensis |
150000 | - |
native protein, gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-aspartate 4-semialdehyde + pyruvate | Caldanaerobacter subterraneus subsp. tengcongensis | - |
dihydrodipicolinate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobacter subterraneus subsp. tengcongensis | Q8RBI5 | DSM 15242T, JCM11007 | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity relatively high even at 30°C, structural model reveals that the active site is well conserved | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-aspartate 4-semialdehyde + pyruvate | - |
Caldanaerobacter subterraneus subsp. tengcongensis | dihydrodipicolinate + H2O | - |
? | |
(S)-aspartate 4-semialdehyde + pyruvate | characterization of thermostable enzyme features, substrate specificity tested, activity measurements at elevated temperature of 60°C | Caldanaerobacter subterraneus subsp. tengcongensis | dihydrodipicolinate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | similar to oligomeric states of other homologous enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at, assay performed at 60°C, structural model reveals that the active site is well conserved | Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | activity analyzed at | Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
3 h at | Caldanaerobacter subterraneus subsp. tengcongensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
specific condensation of pyruvate and (S)-aspartate 4-semialdehyde optimally at | Caldanaerobacter subterraneus subsp. tengcongensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | activity analyzed at | Caldanaerobacter subterraneus subsp. tengcongensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
(S)-lysine | 180 mM, at 60°C | Caldanaerobacter subterraneus subsp. tengcongensis |