Crystallization (Comment) | Organism |
---|---|
structure of MosA protein solved to 1.95 A resolution, data collection and refinement statistics | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxobutyrate | forms a Schiff base with MosA protein, competitive inhibition | Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | Sinorhizobium meliloti | - |
dihydrodipicolinate + H2O | - |
? | |
L-aspartate 4-semialdehyde + pyruvate | Sinorhizobium meliloti L5-30 | - |
dihydrodipicolinate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | Q07607 | L5-30 | - |
Sinorhizobium meliloti L5-30 | Q07607 | L5-30 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
analysis of ability of rhizopines to interact with MosA protein in the presence and absence of methyl donors, no methyltransferase activity observed in the presence of scyllo-inosamine and S-adenosylmethionine (SAM), presence of rhizopine compounds does not affect kinetics of dihydrodipicolinate synthesis | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | - |
Sinorhizobium meliloti | dihydrodipicolinate + H2O | - |
? | |
L-aspartate 4-semialdehyde + pyruvate | isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA) | Sinorhizobium meliloti | dihydrodipicolinate + H2O | - |
? | |
L-aspartate 4-semialdehyde + pyruvate | - |
Sinorhizobium meliloti L5-30 | dihydrodipicolinate + H2O | - |
? | |
L-aspartate 4-semialdehyde + pyruvate | isothermal titration calorimetry (ITC) to study Schiff base formation between an enzyme and its substrate, pyruvate condenses with an active site lysine residue (Lys161 in MosA) | Sinorhizobium meliloti L5-30 | dihydrodipicolinate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Sinorhizobium meliloti |
MosA protein | - |
Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
isothermal titration microcalorimetry at | Sinorhizobium meliloti |