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Literature summary for 4.3.3.7 extracted from

  • Devenish, S.R.; Gerrard, J.A.; Jameson, G.B.; Dobson, R.C.
    The high-resolution structure of dihydrodipicolinate synthase from Escherichia coli bound to its first substrate, pyruvate (2008), Acta Crystallogr. Sect. F, 64, 1092-1095.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 Blue harbouring the plasmid pJG001 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
examination of the specificity of the active site of DHDPS, co-crystallization with the substrate analogue oxaloacetate, data-collection and refinement statistics Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-aspartate 4-semialdehyde + pyruvate Escherichia coli
-
dihydrodipicolinate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6L2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
attempt to examine the specificity of the active site of DHDPS, co-crystallization with the substrate analogue oxaloacetate, solution of the protein structure indicates that pyruvate rather than oxaloacetic acid bounds in the active site, decarboxylation of oxaloacetate not catalysed by DHDPS, rate of pyruvate production independent of DHDPS concentration, indicating that the decarboxylation of oxaloacetate is occurring by a spontaneous and enzyme-independent mechanism, confirmed by kinetic analysis Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-aspartate 4-semialdehyde + pyruvate
-
Escherichia coli dihydrodipicolinate + H2O
-
?
(S)-aspartate 4-semialdehyde + pyruvate biosynthesis of (S)-lysine and meso-diaminopimelate Escherichia coli dihydrodipicolinate + H2O
-
?

Synonyms

Synonyms Comment Organism
DHDPS
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at, measured using a coupled assay with lactate dehydrogenase to detect pyruvate production Escherichia coli