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Literature summary for 4.3.3.7 extracted from

  • Frisch, D.A.; Gengenbach, B.G.; Tommy, A.M.; Sellner, J.M.; Somers, D.A.; Myers, D.E.
    Isolation and characterization of dihydrodipicolinate synthase from maize (1991), Plant Physiol., 96, 444-452.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
L-lysine
-
Zea mays
lysine ethyl ester
-
Zea mays
PMSF
-
Zea mays
S-(2-aminoethyl)-L-cysteine
-
Zea mays
threo-4-hydroxy-L-lysine
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
L-aspartate-4-semialdehyde
-
Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
4 * 37000-38000 + x * 72000, the 72000 Da subunit possibly modifies the structure and kinetic properties, SDS-PAGE Zea mays
130000
-
non-denaturing PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate-4-semialdehyde + pyruvate Zea mays
-
?
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
corn
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Zea mays
-
leaf
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Zea mays

Storage Stability

Storage Stability Organism
4°C, 20 mM bis-Tris propane buffer, pH 8.0, 10 mM pyruvate, 300 mM NaCl, 0.01% NaN3, 3 weeks, 20% loss of activity Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate-4-semialdehyde + pyruvate
-
Zea mays dihydrodipicolinate + H2O
-
?
L-aspartate-4-semialdehyde + pyruvate
-
Zea mays ?
-
?

Subunits

Subunits Comment Organism
? alpha,beta or alpha2beta Zea mays
tetramer 4 * 37000-38000 + x * 72000, the 72000 Da subunit possibly modifies the structure and kinetic properties, SDS-PAGE Zea mays