Literature summary for 4.3.3.6 extracted from

Guedez, G.; Hipp, K.; Windeisen, V.; Derrer, B.; Gengenbacher, M.; B�ttcher, B.; Sinning, I.; Kappes, B.; Tews, I.
Assembly of the eukaryotic PLP-synthase complex from Plasmodium and activation of the Pdx1 enzyme (2012), Structure, 20, 172-184.

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged subunits Pdx1 and Pdx2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL	Plasmodium falciparum
His-tagged subunits Pdx1 and Pdx2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL	Plasmodium berghei

Crystallization (Commentary)

Crystallization (Comment)	Organism
chimeric complex of Pdx2 and Pdx1 from Plasmodium berghei	Plasmodium falciparum
Pdx1, chimeric complex of Pdx1 and Pdx2 from Plasmodium falciparum	Plasmodium berghei

Protein Variants

Protein Variants	Comment	Organism
H196N	Pdx2, catalytically inactive	Plasmodium falciparum
H199N	Pdx2	Plasmodium berghei
L82A	Pdx1	Plasmodium berghei
M103A	Pdx1	Plasmodium berghei
M103F	Pdx1	Plasmodium berghei
M148L	Pdx1	Plasmodium berghei
M19V	Pdx1	Plasmodium berghei
M46I	Pdx1	Plasmodium berghei

Organism

Organism	UniProt	Comment	Textmining
Plasmodium berghei	A0A509AM71	-	-
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+), gel filtration	Plasmodium falciparum
immobilized metal ion affinity chromatography (Ni2+), gel filtration	Plasmodium berghei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00025	-	L82A mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.00031	-	M103A mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.00042	-	M103A mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0007	-	Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium falciparum
0.0008	-	M148L mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0009	-	Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0009	-	Pdx1/Plasmodium berghei Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium falciparum
0.0009	-	Plasmodium falciparum Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0011	-	Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0012	-	L82A mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0013	-	Pdx1/Plasmodium falciparum Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0013	-	Plasmodium berghei Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium falciparum
0.0015	-	M46I mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0018	-	M19V mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.002	-	M19V mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0028	-	M46I mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.0037	-	M148L mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C	Plasmodium berghei
0.087	-	M19V mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.115	-	L82A mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.126	-	M103F mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.15	-	Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.175	-	Pdx1/Plasmodium berghei Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium falciparum
0.175	-	Plasmodium falciparum Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.178	-	M103A mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.229	-	M148L mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.233	-	Pdx1/Plasmodium falciparum Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei
0.233	-	Plasmodium berghei Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium falciparum
0.26	-	Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium falciparum
0.284	-	M46I mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C	Plasmodium berghei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine	-	Plasmodium falciparum	pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate	-	?
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine	-	Plasmodium berghei	pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate	-	?
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+	in the absence of Pdx2	Plasmodium falciparum	pyridoxal 5'-phosphate + 4 H2O + phosphate	-	?
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+	in the absence of Pdx2	Plasmodium berghei	pyridoxal 5'-phosphate + 4 H2O + phosphate	-	?

Subunits

Subunits	Comment	Organism
homododecamer	Pdx1, random association pattern of up to 12 Pdx2 subunits to the Pdx1 dodecamer	Plasmodium falciparum
homododecamer	Pdx1, random association pattern of up to 12 Pdx2 subunits to the Pdx1 dodecamer	Plasmodium berghei

Synonyms

Synonyms	Comment	Organism
PLP-synthase complex	-	Plasmodium falciparum
PLP-synthase complex	-	Plasmodium berghei