Literature summary for 4.3.3.6 extracted from

Zhang, X.; Teng, Y.; Liu, J.; He, Y.; Zhou, K.; Chen, Y.; Zhou, C.
Structural insights into the catalytic mechanism of the yeast pyridoxal 5-phosphate synthase Snz1 (2010), Biochem. J., 432, 445-450.

Search for more literature for 4.3.3.6

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged protein expressed in Escherichia coli BL21 (DE3)	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour diffusion method, 16°C	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
E116A	activity as the wild-type protein	Saccharomyces cerevisiae
K117A	no synthesis of pyridoxal 5'-phosphate, capacity to catalyse dihydroxyacetone phosphate isomerization	Saccharomyces cerevisiae
K148A	no synthesis of pyridoxal 5'-phosphate, capacity to catalyse dihydroxyacetone phosphate isomerization	Saccharomyces cerevisiae
K240A	activity as the wild-type protein	Saccharomyces cerevisiae
R136A/R137A	no synthesis of pyridoxal 5'-phosphate, dihydroxyacetone phosphate isomerization activity as the wild-type protein	Saccharomyces cerevisiae
R164A	completely inactive, dihydroxyacetone phosphate isomerization activity as the wild-type protein	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q03148	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+), gel filtration	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ribulose 5-phosphate + glyceraldehyde 3-phosphate + NH4+	-	Saccharomyces cerevisiae	pyridoxal 5'-phosphate + 4 H2O + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
pyridoxal 5-phosphate synthase Snz1	-	Saccharomyces cerevisiae
Snz1	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)