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Literature summary for 4.3.3.2 extracted from

  • Yamazaki, M.; Asano, T.; Yamazaki, Y.; Sirikantaramas, S.; Sudo, H.; Saito, K.
    Biosynthetic system of camptothecin: An anticancer plant product (2010), Pure Appl. Chem., 82, 213-218.
No PubMed abstract available
Search for more literature for 4.3.3.2

Application

Application Comment Organism
synthesis production of campothecin in hairy root culture of Ophiorrhiza pumila by infection with Agrobacterium tumefaciens. The culture also produces a high level of anthraquinones besides camptothecin. Camptothecin is present not only in hairy root cells, but excreted out to medium at a substantial amount. Camptothecin content in the medium is increased by the presence of a polystyrene resin that absorbs camptothecin Ophiorrhiza pumila

Cloned(Commentary)

Cloned (Comment) Organism
-
 Ophiorrhiza pumila

Protein Variants

Protein Variants Comment Organism
C97S loss of activity Ophiorrhiza pumila
D213E loss of activity Ophiorrhiza pumila
D213N loss of activity Ophiorrhiza pumila
E194D about 30% of wild-type activity Ophiorrhiza pumila
E194V about 55% of wild-type activity Ophiorrhiza pumila
H306L loss of activity Ophiorrhiza pumila
H317L loss of activity Ophiorrhiza pumila
K144L about 124% of wild-type activity Ophiorrhiza pumila
K200L loss of activity Ophiorrhiza pumila
K200R about 90% of wild-type activity Ophiorrhiza pumila
K236L loss of activity Ophiorrhiza pumila
K236R loss of activity Ophiorrhiza pumila
N251S about 115% of wild-type activity Ophiorrhiza pumila
N88S loss of activity Ophiorrhiza pumila
Y63F about 50% of wild-type activity Ophiorrhiza pumila
Y63L about 50% of wild-type activity Ophiorrhiza pumila

Organism

Organism UniProt Comment Textmining
Ophiorrhiza pumila Q94LW9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root hairy root and root, high expression Ophiorrhiza pumila
-
root hairy root and root, high expression Ophiorrhiza pumila
-
stem high expression Ophiorrhiza pumila
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methyltryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
5-hydroxytryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
5-methoxytryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
6-methoxytryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
7-methyltryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
tryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
str
-
 Ophiorrhiza pumila