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Literature summary for 4.3.3.2 extracted from

  • Loris, E.A.; Panjikar, S.; Ruppert, M.; Barleben, L.; Unger, M.; Schuebel, H.; Stoeckigt, J.
    Structure-based engineering of strictosidine synthase: auxiliary for alkaloid libraries (2007), Chem. Biol., 14, 979-985.
    View publication on PubMed
Search for more literature for 4.3.3.2

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of STR1 in complex with strictosidine is solved at a resolution of 3.0 A Rauvolfia serpentina

Protein Variants

Protein Variants Comment Organism
V208A Val208 plays a critical role in substrate recognition at the indole moiety of strictosidine Rauvolfia serpentina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 tryptamine wild-type enzyme Rauvolfia serpentina
0.136
-
 6-fluoro-tryptamine wild-type enzyme Rauvolfia serpentina
0.219
-
 tryptamine mutant V208A Rauvolfia serpentina
0.259
-
 5-fluoro-tryptamine wild-type enzyme Rauvolfia serpentina
0.281
-
 5-methyl-tryptamine mutant V208A Rauvolfia serpentina
0.307
-
 6-methoxy-tryptamine mutant V208A Rauvolfia serpentina
0.356
-
 6-fluoro-tryptamine mutant V208A Rauvolfia serpentina
0.393
-
 6-methyl-tryptamine wild-type enzyme Rauvolfia serpentina
0.762
-
 6-methyl-tryptamine mutant V208A Rauvolfia serpentina
0.844
-
 5-hydroxy-tryptamine mutant V208A Rauvolfia serpentina
0.962
-
 6-methoxy-tryptamine wild-type enzyme Rauvolfia serpentina
1.302
-
 5-fluoro-tryptamine mutant V208A Rauvolfia serpentina
2.255
-
 5-hydroxy-tryptamine wild-type enzyme Rauvolfia serpentina
3.592
-
 5-methoxy-tryptamine mutant V208A Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-alpha(S)-strictosidine + H2O Rauvolfia serpentina
-
 tryptamine + secologanin
-
 ?

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-alpha(S)-strictosidine + H2O
-
 Rauvolfia serpentina tryptamine + secologanin
-
 ?
5-fluoro-tryptamine + H2O
-
 Rauvolfia serpentina ?
-
 ?
5-hydroxy-tryptamine + H2O
-
 Rauvolfia serpentina ?
-
 ?
5-methoxy-tryptamine + H2O
-
 Rauvolfia serpentina ?
-
 ?
5-methyl-tryptamine + H2O
-
 Rauvolfia serpentina ?
-
 ?
6-fluoro-tryptamine + H2O
-
 Rauvolfia serpentina ?
-
 ?
6-methoxy-tryptamine + H2O
-
 Rauvolfia serpentina ?
-
 ?
6-methyl-tryptamine + H2O
-
 Rauvolfia serpentina ?
-
 ?

Synonyms

Synonyms Comment Organism
STR1
-
 Rauvolfia serpentina
strictosidine synthase
-
 Rauvolfia serpentina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
 5-hydroxy-tryptamine wild-type enzyme Rauvolfia serpentina
0.03 0.55 5-methyl-tryptamine mutant V208A Rauvolfia serpentina
0.057 0.65 tryptamine wild-type enzyme Rauvolfia serpentina
0.057 0.65 6-methyl-tryptamine mutant V208A Rauvolfia serpentina
0.21
-
 5-methoxy-tryptamine mutant V208A Rauvolfia serpentina
0.52
-
 6-fluoro-tryptamine mutant V208A Rauvolfia serpentina
0.64
-
 tryptamine mutant V208A Rauvolfia serpentina
0.97
-
 6-methoxy-tryptamine mutant V208A Rauvolfia serpentina
0.99
-
 5-hydroxy-tryptamine mutant V208A Rauvolfia serpentina
2.32
-
 6-methyl-tryptamine wild-type enzyme Rauvolfia serpentina
3.4
-
 5-fluoro-tryptamine wild-type enzyme Rauvolfia serpentina
3.8
-
 5-fluoro-tryptamine mutant V208A Rauvolfia serpentina
5.32
-
 6-methoxy-tryptamine wild-type enzyme Rauvolfia serpentina
6.56
-
 5-methyl-tryptamine mutant V208A Rauvolfia serpentina
10.65
-
 tryptamine wild-type enzyme Rauvolfia serpentina
10.95
-
 6-methyl-tryptamine mutant V208A Rauvolfia serpentina
13.63
-
 6-fluoro-tryptamine mutant V208A Rauvolfia serpentina
16.66
-
 6-methoxy-tryptamine mutant V208A Rauvolfia serpentina
16.9
-
 6-fluoro-tryptamine wild-type enzyme Rauvolfia serpentina
18.12
-
 5-hydroxy-tryptamine mutant V208A Rauvolfia serpentina
21.24
-
 5-fluoro-tryptamine mutant V208A Rauvolfia serpentina
23.37
-
 6-fluoro-tryptamine wild-type enzyme Rauvolfia serpentina
37.46
-
 5-fluoro-tryptamine wild-type enzyme Rauvolfia serpentina
54.09
-
 tryptamine mutant V208A Rauvolfia serpentina
79.66
-
 5-methoxy-tryptamine mutant V208A Rauvolfia serpentina
562.1
-
 5-hydroxy-tryptamine wild-type enzyme Rauvolfia serpentina