BRENDA - Enzyme Database show
show all sequences of 4.3.3.2

Purification and characterization of strictosidine synthase from a suspension culture of Cinchona robusta

Stevens, L.H.; Giroud, C.; Pennings, E.J.M.; Verpoorte, R.; Phytochemistry 33, 99-106 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-alpha(S)-strictosidine
-
Cinchona robusta
chinconine
-
Cinchona robusta
cinchonidine
-
Cinchona robusta
corynantheal
-
Cinchona robusta
Cu2+
-
Cinchona robusta
Dihydroquinine
-
Cinchona robusta
additional information
no substrate inhibition
Cinchona robusta
additional information
no substrate inhibition
Catharanthus roseus
quinidine
-
Cinchona robusta
Quinine
-
Cinchona robusta
Tris
-
Cinchona robusta
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
tryptamine
isoform I b
Cinchona robusta
0.003
-
tryptamine
isoform II b
Cinchona robusta
0.008
-
tryptamine
isoform I a
Cinchona robusta
0.015
-
tryptamine
isoform II a
Cinchona robusta
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
SDS-PAGE, isoform I b and II b
Cinchona robusta
35000
-
SDS-PAGE, isoforms I a and II a
Cinchona robusta
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tryptamine + secologanin
Cinchona robusta
involved in synthesis of monoterpenoid indole alkaloides
3-alpha(S)-strictosidine
-
Cinchona robusta
?
tryptamine + secologanin
Catharanthus roseus
involved in synthesis of monoterpenoid indole alkaloides
3-alpha(S)-strictosidine
-
Catharanthus roseus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
-
-
Cinchona robusta
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Catharanthus roseus
glycoprotein
all isoforms
Cinchona robusta
Purification (Commentary)
Commentary
Organism
hydrophobic interaction chromatography used for purification
Cinchona robusta
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Cinchona robusta
-
cell culture
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-methoxytryptamine + secologanin
10% of activity
648723
Cinchona robusta
10-methoxystrictosidine
-
648723
Cinchona robusta
?
additional information
5-methoxytryptamine is not utilized
648723
Catharanthus roseus
?
-
-
-
-
tryptamine + secologanin
-
648723
Cinchona robusta
3-alpha(S)-strictosidine
-
648723
Cinchona robusta
?
tryptamine + secologanin
-
648723
Catharanthus roseus
3-alpha(S)-strictosidine
-
648723
Catharanthus roseus
?
tryptamine + secologanin
involved in synthesis of monoterpenoid indole alkaloides
648723
Cinchona robusta
3-alpha(S)-strictosidine
-
648723
Cinchona robusta
?
tryptamine + secologanin
involved in synthesis of monoterpenoid indole alkaloides
648723
Catharanthus roseus
3-alpha(S)-strictosidine
-
648723
Catharanthus roseus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8.5
-
Cinchona robusta
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.5
50% activity
Cinchona robusta
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.007
-
Quinine
-
Cinchona robusta
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-alpha(S)-strictosidine
-
Cinchona robusta
chinconine
-
Cinchona robusta
cinchonidine
-
Cinchona robusta
corynantheal
-
Cinchona robusta
Cu2+
-
Cinchona robusta
Dihydroquinine
-
Cinchona robusta
additional information
no substrate inhibition
Cinchona robusta
additional information
no substrate inhibition
Catharanthus roseus
quinidine
-
Cinchona robusta
Quinine
-
Cinchona robusta
Tris
-
Cinchona robusta
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.007
-
Quinine
-
Cinchona robusta
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
tryptamine
isoform I b
Cinchona robusta
0.003
-
tryptamine
isoform II b
Cinchona robusta
0.008
-
tryptamine
isoform I a
Cinchona robusta
0.015
-
tryptamine
isoform II a
Cinchona robusta
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
SDS-PAGE, isoform I b and II b
Cinchona robusta
35000
-
SDS-PAGE, isoforms I a and II a
Cinchona robusta
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tryptamine + secologanin
Cinchona robusta
involved in synthesis of monoterpenoid indole alkaloides
3-alpha(S)-strictosidine
-
Cinchona robusta
?
tryptamine + secologanin
Catharanthus roseus
involved in synthesis of monoterpenoid indole alkaloides
3-alpha(S)-strictosidine
-
Catharanthus roseus
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Catharanthus roseus
glycoprotein
all isoforms
Cinchona robusta
Purification (Commentary) (protein specific)
Commentary
Organism
hydrophobic interaction chromatography used for purification
Cinchona robusta
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Cinchona robusta
-
cell culture
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-methoxytryptamine + secologanin
10% of activity
648723
Cinchona robusta
10-methoxystrictosidine
-
648723
Cinchona robusta
?
additional information
5-methoxytryptamine is not utilized
648723
Catharanthus roseus
?
-
-
-
-
tryptamine + secologanin
-
648723
Cinchona robusta
3-alpha(S)-strictosidine
-
648723
Cinchona robusta
?
tryptamine + secologanin
-
648723
Catharanthus roseus
3-alpha(S)-strictosidine
-
648723
Catharanthus roseus
?
tryptamine + secologanin
involved in synthesis of monoterpenoid indole alkaloides
648723
Cinchona robusta
3-alpha(S)-strictosidine
-
648723
Cinchona robusta
?
tryptamine + secologanin
involved in synthesis of monoterpenoid indole alkaloides
648723
Catharanthus roseus
3-alpha(S)-strictosidine
-
648723
Catharanthus roseus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8.5
-
Cinchona robusta
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.5
50% activity
Cinchona robusta
Other publictions for EC 4.3.3.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749267
Sharma
Overexpression of tryptophan ...
Catharanthus roseus
Protoplasma
255
1281-1294
2018
-
1
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4
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749356
Zou
An atypical strictosidine syn ...
Oryza sativa
Sci. Rep.
7
6863
2017
-
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-
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2
-
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3
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6
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2
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6
-
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2
2
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743812
Pandey
Fungal endophytes of Catharan ...
Catharanthus roseus
Sci. Rep.
6
26583
2016
-
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-
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6
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-
1
1
1
1
-
-
730920
Verma
Over-expression of Catharanthu ...
Catharanthus roseus
Protoplasma
252
373-381
2015
-
-
1
-
1
-
-
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-
-
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-
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5
-
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1
1
-
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746441
Cui
Co-overexpression of geraniol ...
Catharanthus roseus
Sci. Rep.
5
8227
2015
-
2
1
-
-
-
-
-
-
-
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3
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747618
Zhu
Using strictosidine synthase ...
Rauvolfia serpentina
Curr. Med. Chem.
22
1880-1888
2015
-
1
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1
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729396
Fischereder
Engineering strictosidine synt ...
Rauvolfia serpentina
Bioorg. Med. Chem.
22
5633-5637
2014
-
-
1
-
1
-
-
-
-
-
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1
-
3
-
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2
1
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1
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1
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1
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-
2
1
-
-
-
-
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-
-
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3
3
-
-
-
729484
Vera-Reyes
Strictosidine-related enzymes ...
Uncaria tomentosa
Biotechnol. Prog.
29
621-630
2013
-
-
1
-
-
-
-
-
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1
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2
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2
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2
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1
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1
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1
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1
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2
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2
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1
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1
-
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1
2
2
1
-
-
715978
Kusari
Effect of artificial reconstit ...
Camptotheca acuminata
J. Nat. Prod.
74
764-775
2011
-
1
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-
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-
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1
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1
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703154
Yang
Improved expression of His(6)- ...
Rauvolfia serpentina
Chem. Biodivers.
7
860-870
2010
-
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1
-
-
-
-
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1
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3
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1
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1
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1
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2
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1
-
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1
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1
1
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705863
Stockigt
Construction and expression of ...
Rauvolfia serpentina
Nat. Prod. Res.
24
759-766
2010
-
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1
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4
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714624
Guirimand
Strictosidine activation in Ap ...
Catharanthus roseus
BMC Plant Biol.
10
182
2010
-
-
-
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1
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6
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716888
Yamazaki
-
Biosynthetic system of camptot ...
Ophiorrhiza pumila
Pure Appl. Chem.
82
213-218
2010
-
1
1
-
16
-
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1
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3
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6
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16
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3
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6
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694616
Sohani
Phylogenetic and transcription ...
Arabidopsis thaliana
Plant Biol.
11
105-117
2009
1
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1
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5
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1
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5
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5
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5
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705628
Lu
Molecular characterization and ...
Ophiorrhiza japonica
Mol. Biol. Rep.
36
1845-1852
2009
-
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4
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2
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2
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706802
Bernhardt
Synthesis and biochemical eval ...
Rauvolfia serpentina
Tetrahedron Lett.
50
7118-7120
2009
-
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1
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1
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8
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1
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8
-
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680382
Maresh
Strictosidine synthase: mechan ...
Catharanthus roseus, Rauvolfia serpentina
J. Am. Chem. Soc.
130
710-723
2008
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2
1
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2
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3
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2
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2
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1
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2
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1
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1
1
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2
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2
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2
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2
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2
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694743
Stoeckigt
3D-Structure and function of s ...
Catharanthus roseus, Rauvolfia serpentina
Plant Physiol. Biochem.
46
340-355
2008
-
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1
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4
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2
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4
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1
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30
1
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4
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695121
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Molecular cloning and characte ...
Rauvolfia verticillata
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2008
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679175
Bernhardt
Rapid identification of enzyme ...
Catharanthus roseus
Chem. Biol.
14
888-897
2007
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1
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6
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9
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679176
Loris
Structure-based engineering of ...
Rauvolfia serpentina
Chem. Biol.
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979-985
2007
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14
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8
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26
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664326
McCoy
Substrate specificity of stric ...
Escherichia coli
Bioorg. Med. Chem. Lett.
16
2475-2478
2006
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1
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9
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9
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666582
Ma
The Structure of Rauvolfia ser ...
Rauvolfia serpentina
Plant Cell
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2006
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2
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3
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8
8
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7
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679162
Chen
Redesign of a central enzyme i ...
Catharanthus roseus
Chem. Biol.
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2006
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1
1
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14
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663507
Koepke
Crystallization and preliminar ...
Rauvolfia serpentina
Acta Crystallogr. Sect. D
61
690-693
2005
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2
1
1
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664183
Ma
Crystallization and preliminar ...
Rauvolfia serpentina
Biochim. Biophys. Acta
1702
121-124
2004
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2
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1
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5
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1
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648730
Yamazaki
Metabolite profiling of alkalo ...
Ophiorrhiza pumila
Phytochemistry
62
461-470
2003
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1
2
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648729
Whitmer
-
Effect of precursor feeding on ...
Catharanthus roseus
Plant Cell Tissue Organ Cult.
69
85-93
2002
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1
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648728
Pasquali
The promoter of the strictosid ...
Catharanthus roseus
Plant Mol. Biol.
39
1299-1310
1999
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1
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4
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648727
Canel
Effects of over-expression of ...
Catharanthus roseus
Planta
205
414-419
1998
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648726
Hallard
Suspension cultured transgenic ...
Catharanthus roseus
Plant Cell Rep.
17
50-54
1997
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1
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1
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648725
de Waal
Strictosidine synthase from Ca ...
Catharanthus roseus
Biochem. J.
306
571-580
1995
1
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4
1
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1
1
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-
393871
Kutchan
Heterologous expression of the ...
Rauvolfia serpentina
Phytochemistry
35
353-360
1994
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1
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2
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5
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1
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1
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1
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648723
Stevens
-
Purification and characterizat ...
Catharanthus roseus, Cinchona robusta
Phytochemistry
33
99-106
1993
-
-
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11
4
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2
2
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2
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2
1
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2
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6
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1
1
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1
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11
1
4
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2
2
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2
1
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2
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6
-
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1
1
-
-
-
-
-
-
-
-
648721
Roessner
Purification of an indole alka ...
Catharanthus roseus
Protein Expr. Purif.
3
295-300
1992
-
-
-
-
-
-
-
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2
1
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3
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1
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2
1
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1
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1
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2
1
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-
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-
-
648722
Bracher
Strictosidine synthase from Ra ...
Rauvolfia mannii, Rauvolfia serpentina
Arch. Biochem. Biophys.
294
717-723
1992
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2
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2
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8
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4
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4
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4
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4
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-
-
-
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-
-
-
-
648720
Pennings
Assay of trictosidine synthase ...
Catharanthus roseus, Tabernaemontana orientalis
Anal. Biochem.
176
412-415
1989
-
-
-
-
-
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-
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2
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5
-
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1
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1
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1
4
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2
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1
-
1
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648719
Hampp
-
Homogeneous strictosidine synt ...
Rauvolfia serpentina
Phytochemistry
27
3811-3815
1988
-
-
-
-
-
1
-
2
-
-
2
1
1
1
-
1
1
-
-
1
1
2
2
1
1
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
2
1
1
-
1
1
-
1
1
2
2
1
1
-
1
1
1
1
-
-
-
-
-
-
-
-
648717
Skinner
-
Tryptophan decarboxylase, stri ...
Cinchona calisaya
Phytochemistry
26
721-725
1987
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
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-
-
-
1
-
-
3
-
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-
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-
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-
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2
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1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648718
Pfitzner
-
Isoation and immobilization of ...
Catharanthus roseus
Methods Enzymol.
136
342-350
1987
-
1
-
-
-
1
-
-
-
-
-
1
-
1
-
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1
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-
1
1
-
2
1
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1
-
2
2
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-
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-
-
1
-
-
-
-
1
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-
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-
1
-
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1
-
1
1
-
2
1
-
-
1
-
2
2
-
-
-
-
-
-
-
-
648716
Pfitzner
Immobilization of strictosidin ...
Catharanthus roseus
Planta Med.
46
10-14
1982
-
1
-
-
-
1
1
4
-
-
-
1
-
3
-
-
1
-
-
2
-
1
2
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-
-
1
-
2
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-
-
-
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-
1
-
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-
1
-
1
-
4
-
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-
1
-
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-
1
-
2
-
1
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
648713
Treimer
Purification and properties of ...
Amsonia orientalis, Amsonia salicifolia, Amsonia tabernaemontana, Catharanthus longifolius, Catharanthus pusillus, Catharanthus roseus, Catharanthus trichophyllus, Ervatamia divaricatum, Ochrosia elliptica, Rauvolfia verticillata, Rauvolfia vomitoria, Stemmadenia tomentosa var. palmeri, Vinca major, Vinca minor, Voacanga africana
Eur. J. Biochem.
101
225-233
1979
-
-
-
-
-
2
10
4
1
-
1
15
-
18
-
-
1
-
-
10
1
1
38
-
15
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
4
1
-
1
15
-
-
-
1
-
10
1
1
38
-
15
-
-
-
4
1
-
-
-
-
-
-
-
-
648714
Mizukami
Purification and properties of ...
Catharanthus roseus
Biochemistry
18
3760-3763
1979
-
-
-
-
-
-
-
2
-
-
1
1
-
1
-
-
1
-
-
1
1
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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2
-
-
1
1
-
-
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1
-
1
1
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
648715
Treimer
-
Strictosidine synthase from ce ...
Amsonia orientalis, Amsonia salicifolia, Catharanthus roseus, Ochrosia elliptica, Rauvolfia vomitoria, Stemmadenia tomentosa var. palmeri, Vinca major, Vinca minor, Voacanga africana
FEBS Lett.
97
159-162
1979
-
-
-
-
-
-
8
2
-
-
-
9
-
9
-
-
-
-
-
8
-
8
18
8
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
2
-
-
-
9
-
-
-
-
-
8
-
8
18
8
1
-
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-
1
-
-
-
-
-
-
-
-
-