BRENDA - Enzyme Database show
show all sequences of 4.3.3.1

Gene cloning and expression of a 3-ketovalidoxylamine C-N-lyase from Flavobacterium saccharophilum IFO 13984

Kim, J.; Joo, J.; Kim, S.; Yoo, Y.; Biotechnol. Bioprocess Eng. 16, 366-373 (2011)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Flavobacterium saccharophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40878, calculated, x * 40000, SDS-PAGE
Flavobacterium saccharophilum
40878
-
x * 40878, calculated, x * 40000, SDS-PAGE
Flavobacterium saccharophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Flavobacterium saccharophilum
DI109203
-
-
Flavobacterium saccharophilum IFO 13984
DI109203
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
putative N-terminal signal peptide, cleavage site is predicted between position 29 and 30
Flavobacterium saccharophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum
(1S,2S,3R,6S)-6-amino-4-(hydroxymethyl)cyclohex-4-ene-1,2,3-triol + (5R,6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one
i.e. valienamine
-
-
?
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum IFO 13984
(1S,2S,3R,6S)-6-amino-4-(hydroxymethyl)cyclohex-4-ene-1,2,3-triol + (5R,6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one
i.e. valienamine
-
-
?
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum
(6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohexa-2,4-dien-1-one + (1R,2S,3S,4R,6R)-4-amino-6-(hydroxymethyl)cyclohexane-1,2,3-triol
i.e. validamine
-
-
?
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum IFO 13984
(6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohexa-2,4-dien-1-one + (1R,2S,3S,4R,6R)-4-amino-6-(hydroxymethyl)cyclohexane-1,2,3-triol
i.e. validamine
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40878, calculated, x * 40000, SDS-PAGE
Flavobacterium saccharophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Flavobacterium saccharophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40878, calculated, x * 40000, SDS-PAGE
Flavobacterium saccharophilum
40878
-
x * 40878, calculated, x * 40000, SDS-PAGE
Flavobacterium saccharophilum
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
putative N-terminal signal peptide, cleavage site is predicted between position 29 and 30
Flavobacterium saccharophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum
(1S,2S,3R,6S)-6-amino-4-(hydroxymethyl)cyclohex-4-ene-1,2,3-triol + (5R,6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one
i.e. valienamine
-
-
?
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum IFO 13984
(1S,2S,3R,6S)-6-amino-4-(hydroxymethyl)cyclohex-4-ene-1,2,3-triol + (5R,6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one
i.e. valienamine
-
-
?
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum
(6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohexa-2,4-dien-1-one + (1R,2S,3S,4R,6R)-4-amino-6-(hydroxymethyl)cyclohexane-1,2,3-triol
i.e. validamine
-
-
?
3-ketovalidoxylamine A
-
714560
Flavobacterium saccharophilum IFO 13984
(6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohexa-2,4-dien-1-one + (1R,2S,3S,4R,6R)-4-amino-6-(hydroxymethyl)cyclohexane-1,2,3-triol
i.e. validamine
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40878, calculated, x * 40000, SDS-PAGE
Flavobacterium saccharophilum
Other publictions for EC 4.3.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714560
Kim
-
Gene cloning and expression of ...
Flavobacterium saccharophilum, Flavobacterium saccharophilum IFO 13984
Biotechnol. Bioprocess Eng.
16
366-373
2011
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4
1
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701773
Zhang
Purification and Characterizat ...
Stenotrophomonas maltophilia
Appl. Biochem. Biotechnol.
162
966-74
2010
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3
1
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2
1
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2
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1
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701595
Zhang
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Study on optimal production of ...
Stenotrophomonas maltophilia
Afr. J. Biotechnol.
8
5482-5488
2009
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677731
Zhang
Preparation of 3-ketovalidoxyl ...
Stenotrophomonas maltophilia
Appl. Microbiol. Biotechnol.
73
1275-1281
2007
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648712
Takeuchi
Chemical modification by 2,4,6 ...
Flavobacterium saccharophilum
Chem. Pharm. Bull.
38
1419-1420
1990
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648711
Takeuchi
Fluorometric studies on the ro ...
Flavobacterium saccharophilum
Chem. Pharm. Bull.
36
3540-3545
1988
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648710
Takeuchi
Chemical modification by dieth ...
Flavobacterium saccharophilum
J. Biochem.
99
1571-1577
1986
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5
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5
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648709
Takeuchi
Purification and properties of ...
Flavobacterium saccharophilum
J. Biochem.
98
1631-1638
1985
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1
9
3
2
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1
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2
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7
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9
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7
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648708
Asano
Microbial degradation of valid ...
Flavobacterium saccharophilum
J. Antibiot.
37
859-867
1984
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