BRENDA - Enzyme Database show
show all sequences of 4.3.3.1

Purification and Characterization of 3-Ketovalidoxylamine A C-N Lyase Produced by Stenotrophomonas maltrophilia

Zhang, J.F.; Zheng, Y.G.; Shen, Y.C.; Appl. Biochem. Biotechnol. 162, 966-74 (2010)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2',2'',2'''-(1,2-ethanediyldinitrilo)tetraacetic acid
-
Stenotrophomonas maltophilia
4-nitroaniline
inhibition at low concentration
Stenotrophomonas maltophilia
Zn2+
-
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
4-nitrophenyl-3-ketovalidamine
-
Stenotrophomonas maltophilia
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
promoting, reverses complete enzyme inhibition by EDTA
Stenotrophomonas maltophilia
Zn2+
inhibitor
Stenotrophomonas maltophilia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
SDS-PAGE
Stenotrophomonas maltophilia
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Stenotrophomonas maltophilia
-
-
-
Purification (Commentary)
Commentary
Organism
367.5fold by column chromatography
Stenotrophomonas maltophilia
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.94
-
after complete purification
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl-3-ketovalidamine
assay at pH 7.0
701773
Stenotrophomonas maltophilia
4-nitroaniline + ?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Stenotrophomonas maltophilia
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
enzyme sensitive to heat
Stenotrophomonas maltophilia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Stenotrophomonas maltophilia
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
10.5
-
Stenotrophomonas maltophilia
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Stenotrophomonas maltophilia
precast gel
-
7
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2',2'',2'''-(1,2-ethanediyldinitrilo)tetraacetic acid
-
Stenotrophomonas maltophilia
4-nitroaniline
inhibition at low concentration
Stenotrophomonas maltophilia
Zn2+
-
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
4-nitrophenyl-3-ketovalidamine
-
Stenotrophomonas maltophilia
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
promoting, reverses complete enzyme inhibition by EDTA
Stenotrophomonas maltophilia
Zn2+
inhibitor
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
SDS-PAGE
Stenotrophomonas maltophilia
Purification (Commentary) (protein specific)
Commentary
Organism
367.5fold by column chromatography
Stenotrophomonas maltophilia
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.94
-
after complete purification
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl-3-ketovalidamine
assay at pH 7.0
701773
Stenotrophomonas maltophilia
4-nitroaniline + ?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Stenotrophomonas maltophilia
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
enzyme sensitive to heat
Stenotrophomonas maltophilia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Stenotrophomonas maltophilia
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
10.5
-
Stenotrophomonas maltophilia
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Stenotrophomonas maltophilia
precast gel
-
7
Other publictions for EC 4.3.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714560
Kim
-
Gene cloning and expression of ...
Flavobacterium saccharophilum, Flavobacterium saccharophilum IFO 13984
Biotechnol. Bioprocess Eng.
16
366-373
2011
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701773
Zhang
Purification and Characterizat ...
Stenotrophomonas maltophilia
Appl. Biochem. Biotechnol.
162
966-74
2010
-
-
-
-
-
-
3
1
-
2
1
-
-
2
-
-
1
-
-
1
1
-
1
-
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
3
-
1
-
2
1
-
-
-
-
1
-
1
1
-
1
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
701595
Zhang
-
Study on optimal production of ...
Stenotrophomonas maltophilia
Afr. J. Biotechnol.
8
5482-5488
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
677731
Zhang
Preparation of 3-ketovalidoxyl ...
Stenotrophomonas maltophilia
Appl. Microbiol. Biotechnol.
73
1275-1281
2007
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648712
Takeuchi
Chemical modification by 2,4,6 ...
Flavobacterium saccharophilum
Chem. Pharm. Bull.
38
1419-1420
1990
-
-
-
-
-
-
1
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
648711
Takeuchi
Fluorometric studies on the ro ...
Flavobacterium saccharophilum
Chem. Pharm. Bull.
36
3540-3545
1988
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648710
Takeuchi
Chemical modification by dieth ...
Flavobacterium saccharophilum
J. Biochem.
99
1571-1577
1986
-
-
-
-
-
-
1
-
-
1
2
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
2
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648709
Takeuchi
Purification and properties of ...
Flavobacterium saccharophilum
J. Biochem.
98
1631-1638
1985
-
-
-
-
-
1
9
3
2
1
1
-
-
2
-
-
1
-
-
1
1
1
7
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
3
2
1
1
-
-
-
-
1
-
1
1
1
7
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
648708
Asano
Microbial degradation of valid ...
Flavobacterium saccharophilum
J. Antibiot.
37
859-867
1984
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-