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Literature summary for 4.3.2.9 extracted from
- Identification and characterization of {gamma}-glutamylamine cyclotransferase: An enzyme responsible for {gamma}-glutamyl-{epsilon}-lysine catabolism (2010), J. Biol. Chem., 285, 9642-9648.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pGEM-T for sequencing and subsequently into pHUE for expression of the protein in Escherichia coli BL21DE3 cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the structures of GGACT, GGACT in complex with 5-oxoproline, and the E82Q mutant are solved to a resolution of 1.20, 1.20 and 0.98 A, respectively | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E82A | mutant, inactive | Homo sapiens |
| E82Q | mutant, inactive | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17300 | - |
recombinant, expressed in Escherichia coli | Homo sapiens |
| 17330 | - |
theoretical, human A2LD1 | Homo sapiens |
| 18000 | - |
determined by gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (gamma-L-glutamyl)-L-amino acid | Homo sapiens | - |
5-oxoproline + L-amino acid | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9BVM4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-agarose chromatography, subsequent removal of all additional N-terminal residues by cleavage with the catalytic domain of mouse ubiquitin specific protease 2 | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.7 | - |
L-gamma-glutamyl-L-epsilon-lysine | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (gamma-L-glutamyl)-L-amino acid | - |
Homo sapiens | 5-oxoproline + L-amino acid | - |
? | |
| L-gamma-glutamyl-L-epsilon-lysine | - |
Homo sapiens | 5-oxoproline + L-lysine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| A2LD1 | - |
Homo sapiens |
| GGACT | - |
Homo sapiens |
| {gamma}-glutamylamine cyclotransferase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Homo sapiens |
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Release 2023.1 (January 2023)
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