Literature summary for 4.3.2.9 extracted from

  • Meister, A.
    gamma-Glutamylcyclotransferase from rat kidney (1981), Methods Enzymol., 113, 428-445.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-aminoglutaryl-L-1-aminobutyrate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
5-L-Glutamyl-L-alpha-aminobutyrate isoenzyme with pI 5.1, in extract, Km increases 4-10fold during purification Rattus norvegicus
6.6
-
5-L-Glutamyl-L-alpha-aminobutyrate isoenzyme with pI 4.6 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
1 * 27000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme with pI 5.1: ammonium sulfate, heat, Sephadex G-75, DEAE-cellulose, thiol-Sepharose 4B, isoenzyme with pI 4.6: ammonium sulfate, heat, CM-cellulose, Sephadex G-75, DEAE-cellulose, ioselectric focusing, gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Mus musculus
-
kidney
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
158
-
pI 4.6 isoenzyme Rattus norvegicus
181
-
pI 5.1 isoenzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
0°C, pI 5.1-isoform, 3 weeks, 30% loss of activity Rattus norvegicus
0°C, pI 4.6-isoform, at least 2 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-L-amino acid
-
Mus musculus 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-L-amino acid
-
Rattus norvegicus 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-5-L-glutamyl-L-amino acid
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-amino acid
-
?
5-L-glutamyl-5-L-glutamyl-L-alanine high activity Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-alanine
-
?
5-L-glutamyl-5-L-glutamyl-L-glutamine high activity Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-glutamine
-
?
5-L-glutamyl-5-L-glutamyl-glycine high activity Rattus norvegicus 5-oxoproline + 5-L-glutamyl-glycine
-
?
5-L-glutamyl-5-L-glutamyl-L-threonine high activity Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-threonine
-
?
5-L-glutamyl-5-L-glutamyl-L-valine
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-valine
-
?
5-L-glutamyl-5-L-glutamyl-L-leucine
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-leucine
-
?
5-L-glutamyl-5-L-glutamyl-L-tyrosine
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-tyrosine
-
?
5-L-glutamyl-5-L-glutamyl-L-glutamate
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-glutamate
-
?
5-L-glutamyl-5-L-glutamyl-L-phenylalanine
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-phenylalanine
-
?
5-L-glutamyl-5-L-glutamyl-L-lysine
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-lysine
-
?
5-L-glutamyl-5-L-glutamyl-L-proline
-
Rattus norvegicus 5-oxoproline + 5-L-glutamyl-L-proline
-
?
5-L-glutamyl-L-methionine
-
Rattus norvegicus 5-oxoproline + L-methionine
-
?
5-L-glutamyl-L-cysteine
-
Rattus norvegicus 5-oxoproline + L-cysteine
-
?
5-L-glutamyl-L-phenylalanine poor substrate Rattus norvegicus 5-oxoproline + L-phenylalanine
-
?
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate model substrate, in vivo and in vitro Mus musculus 3-methyl-5-oxoproline + L-alpha-aminobutyrate
-
?
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate model substrate, in vivo and in vitro Rattus norvegicus 3-methyl-5-oxoproline + L-alpha-aminobutyrate
-
?
5-L-glutamyl-L-glutamine best substrate Rattus norvegicus 5-oxoproline + L-glutamine
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
-
Rattus norvegicus 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
-
?
5-L-glutamyl-L-alanine
-
Rattus norvegicus 5-L-oxoproline + L-alanine
-
?
5-L-glutamyl-L-alpha-aminobutyrate
-
Mus musculus 5-L-oxoproline + L-alpha-aminobutyrate
-
?
5-L-glutamyl-L-alpha-aminobutyrate
-
Rattus norvegicus 5-L-oxoproline + L-alpha-aminobutyrate
-
?
5-L-glutamyl-L-valine very low activity Rattus norvegicus 5-oxoproline + L-valine
-
?
5-L-glutamyl-L-valine poor substrate Rattus norvegicus 5-oxoproline + L-valine
-
?
5-L-glutamyl-glycine poor substrate Rattus norvegicus 5-oxoproline + glycine
-
?
5-L-glutamyl-L-glutamate
-
Rattus norvegicus 5-oxoproline + L-glutamate
-
?
5-L-glutamyl-L-leucine very low activity Rattus norvegicus 5-oxoproline + L-leucine
-
?
5-L-glutamyl-cysteine
-
Rattus norvegicus 5-oxoproline + L-cysteine
-
?
5-L-glutamyl-L-aspartate
-
Rattus norvegicus 5-oxoproline + L-aspartate
-
?
5-L-glutamyl-L-tyrosine very low activity Rattus norvegicus 5-oxoproline + L-tyrosine
-
?
5-L-glutamyl-L-isoleucine very low activity Rattus norvegicus 5-oxoproline + L-isoleucine
-
?
5-L-glutamyl-L-proline very low activity Rattus norvegicus 5-oxoproline + L-proline
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
two isozymic forms of different pI-values: pI 4.6 and 5.1 Rattus norvegicus
7.5 8
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.46
-
2-aminoglutaryl-L-alpha-aminobutyrate
-
Rattus norvegicus