Literature summary for 4.3.2.9 extracted from

  • Orlowski, M.; Richman, P.G.; Meister, A.
    Isolation and properties of gamma-L-glutamylcyclotransferase from human brain (1969), Biochemistry, 8, 1048-1055.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
5-L-Glutamyl-5-L-glutamyl-4-nitroanilide
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens
-
-
cytoplasm
-
Ovis aries
-
-

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ovis aries
pH 4.2, ammonium sulfate, heat, Sephadex G-75, carboxymethyl cellulose, DEAE-cellulose Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
brain
-
Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, stable Homo sapiens
-20°C, stable Ovis aries
0°C, at least 1 month Homo sapiens
0°C, at least 1 month Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-L-amino acid no activity with LD-, DD- and DL-isomers of 5-L-glutamyl-5-L-glutamyl-4-nitroanilide Homo sapiens 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-L-amino acid no activity with LD-, DD- and DL-isomers of 5-L-glutamyl-5-L-glutamyl-4-nitroanilide Ovis aries 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-L-amino acid no activity with: glutathione, i.e. gamma-L-glutamyl-L-cysteinylglycine Homo sapiens 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-L-amino acid no activity with: glutathione, i.e. gamma-L-glutamyl-L-cysteinylglycine Ovis aries 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-L-amino acid no activity with: 5-glutamyl-4-nitroanilide Homo sapiens 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-L-amino acid no activity with: 5-glutamyl-4-nitroanilide Ovis aries 5-oxoproline + L-amino acid i.e. 2-pyrrolidone-5-carboxylic acid ?
5-L-glutamyl-L-glutamine
-
Homo sapiens 5-oxoproline + L-glutamine
-
?
5-L-glutamyl-L-glutamine
-
Ovis aries 5-oxoproline + L-glutamine
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide best substrate Homo sapiens 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide best substrate Ovis aries 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
-
?
5-L-glutamyl-L-alanine
-
Homo sapiens 5-L-oxoproline + L-alanine
-
?
5-L-glutamyl-L-alanine
-
Ovis aries 5-L-oxoproline + L-alanine
-
?
5-L-glutamyl-L-alpha-aminobutyrate
-
Homo sapiens 5-L-oxoproline + L-alpha-aminobutyrate
-
?
5-L-glutamyl-L-alpha-aminobutyrate
-
Ovis aries 5-L-oxoproline + L-alpha-aminobutyrate
-
?
5-L-glutamyl-glycine
-
Homo sapiens 5-oxoproline + glycine
-
?
5-L-glutamyl-glycine
-
Ovis aries 5-oxoproline + glycine
-
?
5-L-glutamyl-S-methyl-L-cysteine
-
Homo sapiens 5-oxoproline + S-methyl-L-cysteine
-
?
5-L-glutamyl-S-methyl-L-cysteine
-
Ovis aries 5-oxoproline + S-methyl-L-cysteine
-
?
5-L-glutamyl-5-L-glutamyl-4-nitroanilide
-
Homo sapiens 5-oxoproline + 5-L-glutamyl-4-nitroanilide
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
purified enzyme, complete loss of activity after 5 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
Homo sapiens
7.8 8.2
-
Ovis aries