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Literature summary for 4.3.2.6 extracted from

  • Llewellyn, N.M.; Li, Y.; Spencer, J.B.
    Biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain (2007), Chem. Biol., 14, 379-386.
    View publication on PubMed
Search for more literature for 4.3.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18100
-
 1 * 18100, calculated from amino acid sequence Niallia circulans
20093
-
 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans NR3312
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA affinity column chromatography Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gamma-L-glutamyl-butirosin B
-
 Niallia circulans butirosin B + 5-oxoproline
-
 ?
gamma-L-glutamyl-butirosin B
-
 Niallia circulans NR3312 butirosin B + 5-oxoproline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 18100, calculated from amino acid sequence Niallia circulans
monomer 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry Niallia circulans

Synonyms

Synonyms Comment Organism
btrG
-
 Niallia circulans
gamma-glutamyl cyclotransferase
-
 Niallia circulans
GGC
-
 Niallia circulans

General Information

General Information Comment Organism
metabolism the enzyme is responsible for the deprotection of the gamma-L-glutamyl-(S)-4-amino-2-hydroxybutyrate side chain following transfer onto ribostamycin Niallia circulans