BRENDA - Enzyme Database
show all sequences of 4.3.2.5

Drosophila uses two distinct neuropeptide amidating enzymes, dPAL1 and dPAL2

Han, M.; Park, D.; Vanderzalm, P.J.; Mains, R.E.; Eipper, B.A.; Taghert, P.H.; J. Neurochem. 90, 129-141 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Cd2+
;
Drosophila melanogaster
Cd2+
-
Rattus norvegicus
Co2+
;
Drosophila melanogaster
Co2+
-
Rattus norvegicus
Cloned(Commentary)
Commentary
Organism
-
Drosophila melanogaster
-
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM; 1 mM
Drosophila melanogaster
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
alpha-N-Acetyl-Tyr-Val-alpha-hydroxyglycine
-
Rattus norvegicus
0.045
-
alpha-N-Acetyl-Tyr-Val-alpha-hydroxyglycine
-
Drosophila melanogaster
0.238
-
alpha-N-Acetyl-Tyr-Val-alpha-hydroxyglycine
-
Drosophila melanogaster
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
;
Drosophila melanogaster
5783
-
Golgi trans face
-
Drosophila melanogaster
-
-
soluble
-
Drosophila melanogaster
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cd2+
activates the enzyme; activates the enzyme
Drosophila melanogaster
Cd2+
activates the enzyme
Rattus norvegicus
Co2+
activates the enzyme; activates the enzyme
Drosophila melanogaster
Co2+
activates the enzyme
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43900
-
monomer, secreted, rhodopsin-tagged protein, calculated from amino acid sequence
Drosophila melanogaster
44400
-
monomer, secreted, rhodopsin-tagged protein, calculated from amino acid sequence
Drosophila melanogaster
49300
-
x * 49300, rhodopsin-tagged protein, SDS-PAGE
Drosophila melanogaster
51300
-
x * 51300, rhodopsin-tagged protein, SDS-PAGE
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
peptidylamidoglycolate
Drosophila melanogaster
-
peptidyl amide + glyoxylate
-
-
?
peptidylamidoglycolate
Rattus norvegicus
-
peptidyl amide + glyoxylate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
Q9V5E1
-
-
Drosophila melanogaster
Q9W1L5
-
-
Rattus norvegicus
P14925
-
-
Purification (Commentary)
Commentary
Organism
-
Drosophila melanogaster
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
central nervous system
;
Drosophila melanogaster
-
central nervous system
-
Rattus norvegicus
-
neuron
;
Drosophila melanogaster
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-N-acetyl-Tyr-Val-alpha-hydroxyglycine
-
666112
Drosophila melanogaster
alpha-N-acetyl-Tyr-Val-NH2 + glyoxylate
-
-
-
?
alpha-N-acetyl-Tyr-Val-alpha-hydroxyglycine
-
666112
Rattus norvegicus
alpha-N-acetyl-Tyr-Val-NH2 + glyoxylate
-
-
-
?
peptidylamidoglycolate
-
666112
Drosophila melanogaster
peptidyl amide + glyoxylate
-
-
-
?
peptidylamidoglycolate
-
666112
Rattus norvegicus
peptidyl amide + glyoxylate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 49300, rhodopsin-tagged protein, SDS-PAGE; x * 51300, rhodopsin-tagged protein, SDS-PAGE
Drosophila melanogaster
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Drosophila melanogaster
7.5
-
-
Drosophila melanogaster
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Cd2+
-
Drosophila melanogaster
Cd2+
-
Rattus norvegicus
Co2+
-
Drosophila melanogaster
Co2+
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Drosophila melanogaster
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM
Drosophila melanogaster
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
alpha-N-Acetyl-Tyr-Val-alpha-hydroxyglycine
-
Rattus norvegicus
0.045
-
alpha-N-Acetyl-Tyr-Val-alpha-hydroxyglycine
-
Drosophila melanogaster
0.238
-
alpha-N-Acetyl-Tyr-Val-alpha-hydroxyglycine
-
Drosophila melanogaster
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Drosophila melanogaster
5783
-
Golgi trans face
-
Drosophila melanogaster
-
-
soluble
-
Drosophila melanogaster
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cd2+
activates the enzyme
Drosophila melanogaster
Cd2+
activates the enzyme
Rattus norvegicus
Co2+
activates the enzyme
Drosophila melanogaster
Co2+
activates the enzyme
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43900
-
monomer, secreted, rhodopsin-tagged protein, calculated from amino acid sequence
Drosophila melanogaster
44400
-
monomer, secreted, rhodopsin-tagged protein, calculated from amino acid sequence
Drosophila melanogaster
49300
-
x * 49300, rhodopsin-tagged protein, SDS-PAGE
Drosophila melanogaster
51300
-
x * 51300, rhodopsin-tagged protein, SDS-PAGE
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
peptidylamidoglycolate
Drosophila melanogaster
-
peptidyl amide + glyoxylate
-
-
?
peptidylamidoglycolate
Rattus norvegicus
-
peptidyl amide + glyoxylate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Drosophila melanogaster
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
central nervous system
-
Drosophila melanogaster
-
central nervous system
-
Rattus norvegicus
-
neuron
-
Drosophila melanogaster
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-N-acetyl-Tyr-Val-alpha-hydroxyglycine
-
666112
Drosophila melanogaster
alpha-N-acetyl-Tyr-Val-NH2 + glyoxylate
-
-
-
?
alpha-N-acetyl-Tyr-Val-alpha-hydroxyglycine
-
666112
Rattus norvegicus
alpha-N-acetyl-Tyr-Val-NH2 + glyoxylate
-
-
-
?
peptidylamidoglycolate
-
666112
Drosophila melanogaster
peptidyl amide + glyoxylate
-
-
-
?
peptidylamidoglycolate
-
666112
Rattus norvegicus
peptidyl amide + glyoxylate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 49300, rhodopsin-tagged protein, SDS-PAGE
Drosophila melanogaster
?
x * 51300, rhodopsin-tagged protein, SDS-PAGE
Drosophila melanogaster
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Drosophila melanogaster
7.5
-
-
Drosophila melanogaster
Other publictions for EC 4.3.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730684
Yin
Probing the production of amid ...
Mus musculus, Mus musculus C57/BL6J
PLoS ONE
6
e28679
2011
-
-
-
-
-
-
-
-
-
-
-
4
-
61
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
691012
Takahashi
Involvement of metals in enzym ...
Homo sapiens
Biochemistry
48
1654-1662
2009
-
-
-
-
-
-
-
3
-
7
-
1
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
7
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
664714
Park
Ap-let neurons-a peptidergic c ...
Drosophila melanogaster
Dev. Biol.
269
95-108
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666112
Han
Drosophila uses two distinct n ...
Drosophila melanogaster, Rattus norvegicus
J. Neurochem.
90
129-141
2004
4
-
2
-
-
-
1
3
3
4
4
3
-
3
-
-
2
-
-
3
-
-
6
1
-
-
-
-
2
-
-
-
-
-
-
6
-
2
-
-
-
-
-
2
-
3
4
6
4
3
-
-
-
2
-
5
-
-
6
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
648703
Satani
Expression and characterizatio ...
Homo sapiens
Protein Expr. Purif.
28
293-302
2003
-
-
1
-
-
-
2
-
1
-
-
-
-
3
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648704
Kolhekar
Essential features of the cata ...
Rattus norvegicus
Biochemistry
41
12384-12394
2002
-
-
1
-
12
-
6
7
1
1
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
12
-
-
6
-
7
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
648706
Garmendia
Immunocytochemical finding of ...
Homo sapiens, Mus musculus, Rattus norvegicus
J. Histochem. Cytochem.
50
1401-1415
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648705
Moore
Kinetic and inhibition studies ...
Bos taurus, Xenopus laevis
Biochem. J.
341
33-40
1999
-
-
1
-
-
-
5
4
-
-
-
-
-
3
-
-
1
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
5
6
4
-
-
-
-
-
-
-
1
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438594
Francisco
Kinetic mechanism and intrinsi ...
Rattus norvegicus
Biochemistry
37
8244-8252
1998
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438592
Ping
Reaction versus subsite stereo ...
Bos taurus
J. Biol. Chem.
270
29250-29255
1995
-
-
-
-
-
-
3
8
-
-
-
-
-
1
-
-
1
-
-
1
-
-
8
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
8
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648693
Husten
Use of endoproteases to identi ...
Bos taurus, Rattus norvegicus
J. Biol. Chem.
268
9709-9717
1993
-
-
1
-
-
-
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
648694
Ping
-
Tandem stereochemistry of pept ...
Bos taurus
J. Am. Chem. Soc.
114
3998-4000
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438571
Merkler
Recombinant type A rat 75-kDa ...
Rattus norvegicus
Arch. Biochem. Biophys.
289
192-196
1991
-
-
1
-
-
-
-
2
2
-
1
-
-
1
-
-
-
-
-
1
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648692
Katopodis
Functional and structural char ...
Bos taurus
Biochemistry
30
6189-6194
1991
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648695
Husten
The membrane-bound bifunctiona ...
Rattus norvegicus
J. Biol. Chem.
266
17004-17010
1991
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648697
Eipper
Peptidyl-alpha-hydroxyglycine ...
Bos taurus
J. Biol. Chem.
266
7827-7833
1991
-
-
-
-
-
-
5
1
-
-
1
-
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
1
-
-
-
-
1
-
2
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
438567
Perkins
The 108-kDA peptidylglycine al ...
Bos taurus
Biochem. Biophys. Res. Commun.
171
926-932
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438569
Suzuki
Elucidation of amidating react ...
Xenopus laevis
EMBO J.
9
4259-4265
1990
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648691
Katopodis
A novel enzyme from bovine neu ...
Bos taurus
Biochemistry
29
6115-6120
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
2
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648700
Takahashi
Peptidylglycine alpha-amidatin ...
Bos taurus, Rattus norvegicus
Biochem. Biophys. Res. Commun.
169
524-530
1990
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648701
Kato
Two enzymes concerned in pepti ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
172
197-203
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-