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Literature summary for 4.3.2.5 extracted from

  • Husten, E.J.; Tausk, F.A.; Keutmann, H.T.; Eipper, B.A.
    Use of endoproteases to identify catalytic domains, linker regions, and functional interactions in soluble peptidylglycine alpha-amidating monooxygenase (1993), J. Biol. Chem., 268, 9709-9717.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in a human embryonic kidney cell line that lacks regulated secretory granules Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 7.4, with endoprotease Arg-C digested PAM-3 protein Rattus norvegicus
0.037
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 7.4, undigested PAM-3 protein Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
soluble, bifunctional form PAM-3, expression in a human embryonic kidney cell line that lacks regulated secretory granules, EC 1.14.17.3 and EC 4.3.2.5 are contained within the bifunctional peptidylglycine alpha-amidation monooxygenase, PAM
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-hydroxyglycine-extended peptide
-
Rattus norvegicus peptidyl amide + glyoxylate
-
?
alpha-hydroxyglycine-extended peptide
-
Bos taurus peptidyl amide + glyoxylate
-
?
Nalpha-acetyl-Tyr-Val-alpha-hydroxyglycine
-
Rattus norvegicus Nalpha-acetyl-Tyr-Val-NH2 + glyoxylate
-
?
Nalpha-acetyl-Tyr-Val-alpha-hydroxyglycine
-
Bos taurus Nalpha-acetyl-Tyr-Val-NH2 + glyoxylate
-
?

Synonyms

Synonyms Comment Organism
PAL
-
Rattus norvegicus
PAL
-
Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
86
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 7.4, with endoprotease Arg-C digested PAM-3 protein Rattus norvegicus
92
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 7.4, undigested PAM-3 protein Rattus norvegicus