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Literature summary for 4.3.2.3 extracted from
- Degradation of ureidoglycolate in French bean (Phaseolus vulgaris) is catalysed by a ubiquitous ureidoglycolate urea-lyase (2006), Planta, 224, 175-184.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-asparagine | - |
Phaseolus vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, complete inhibition, activity can be restored by Mn2+ and Co2+, slightly by Zn2+ | Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
ureidoglycolate | 50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine | Phaseolus vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Phaseolus vulgaris | |
| Mg2+ | - |
Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
6 * 38000, SDS-PAGE | Phaseolus vulgaris |
| 240000 | - |
SDS-PAGE | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ureidoglycolate | Phaseolus vulgaris | - |
glyoxylate + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
L. cv. Great Northern | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, gel filtration, heat treatment, hydrophobic interaction chromatography, immunoaffinity chromatography | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | all tissues | Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.439 | - |
50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ureidoglycolate | - |
Phaseolus vulgaris | glyoxylate + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 38000, SDS-PAGE | Phaseolus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ureidoglycolate urea-lyase | - |
Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Phaseolus vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
ureidoglycolate | 50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine | Phaseolus vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Phaseolus vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Phaseolus vulgaris |
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