Literature summary for 4.3.2.2 extracted from

Kozlov, G.; Nguyen, L.; Pearsall, J.; Gehring, K.
The structure of phosphate-bound Escherichia coli adenylosuccinate lyase identifies His171 as a catalytic acid (2009), Acta Crystallogr. Sect. F, 65, 857-861.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 1.4 M sodium/potassium phosphate pH 7.5 and 4% (v/v) glycerol	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
200000	-	gel filtration	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide	Escherichia coli	-	5-aminoimidazole-4-carboxamide ribonucleotide + fumarate	-	?
succinyladenosine monophosphate	Escherichia coli	-	AMP + fumarate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q8X737	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide	-	Escherichia coli	5-aminoimidazole-4-carboxamide ribonucleotide + fumarate	-	?
succinyladenosine monophosphate	-	Escherichia coli	AMP + fumarate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	x-ray crystallography	Escherichia coli

Synonyms

Synonyms	Comment	Organism
ASL	-	Escherichia coli

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Release 2023.1 (January 2023)