Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D65R | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
D65R/D69E | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
D69E | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
E80D/D87E | australian patient with autism | Homo sapiens |
I62D | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
I62D/D65R | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
I62D/D65R/D69E | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
I62D/D69E | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
I62E | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
I62E/D65R | "humanized" normal enzyme | Bacillus subtilis |
I62E/D65R/D69E | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
I62E/D69E | mutant with differences in thermal stability, catalytic activity and substrate binding | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
adenylsuccinate | mutant D69E, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.002 | - |
adenylsuccinate | mutant D65R/D69E, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.004 | - |
adenylsuccinate | 50 mM HEPES, pH 7.0, 25°C | Homo sapiens | |
0.0043 | - |
adenylsuccinate | mutant I62E/D69E, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0046 | - |
adenylsuccinate | mutant D65R, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0061 | - |
adenylsuccinate | mutant I62E/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0107 | - |
adenylsuccinate | mutant I62D/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0175 | - |
adenylsuccinate | mutant I62E, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0177 | - |
adenylsuccinate | mutant I62D/D69E, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0181 | - |
adenylsuccinate | mutant I62E/D65R, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0488 | - |
adenylsuccinate | mutant I62D/D65R, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis | |
0.0551 | - |
adenylsuccinate | mutant I62D, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
mutant D69E, light scattering method, 0.25 mg/ml | Bacillus subtilis |
159000 | - |
mutant I62E/D65R/D69E, light scattering method, 0.25 mg/ml | Bacillus subtilis |
173000 | - |
mutant I62E, light scattering method, 0.25 mg/ml | Bacillus subtilis |
176000 | - |
mutant I62D, light scattering method, 0.25 mg/ml | Bacillus subtilis |
181000 | - |
light scattering method, 0.25 mg/ml | Homo sapiens |
209000 | - |
mutant I62E/D65R, native PAGE | Bacillus subtilis |
210000 | - |
native PAGE | Homo sapiens |
217000 | - |
mutant I62D/D65R, native PAGE | Bacillus subtilis |
234000 | - |
mutant I62D/D65R/D69E, light scattering method, 0.25 mg/ml | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide | Bacillus subtilis | - |
5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide | Homo sapiens | - |
5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
adenylosuccinate | Bacillus subtilis | - |
AMP + fumarate | - |
? | |
adenylosuccinate | Homo sapiens | - |
AMP + fumarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.063 | - |
mutant I62D/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.069 | - |
mutant I62D/D65R, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.072 | - |
mutant I62D/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.094 | - |
mutant I62D/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.18 | - |
mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.24 | - |
mutant I62D/D65R + I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.33 | - |
mutant I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.46 | - |
mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.53 | - |
mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.54 | - |
mutant I62E/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
0.97 | - |
mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
1.07 | - |
adenylsuccinate and succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Homo sapiens |
1.09 | - |
mutant I62D/D65R, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
1.29 | - |
mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
1.3 | - |
mutant I62D/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
1.4 | - |
mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Bacillus subtilis |
1.58 | - |
adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C | Homo sapiens |
1.7 | - |
succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide | - |
Bacillus subtilis | 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide | - |
Homo sapiens | 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
adenylosuccinate | - |
Bacillus subtilis | AMP + fumarate | - |
? | |
adenylosuccinate | - |
Homo sapiens | AMP + fumarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Bacillus subtilis |
homotetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ASL | - |
Bacillus subtilis |
ASL | - |
Homo sapiens |