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Literature summary for 4.3.2.2 extracted from

  • Barshop, B.A.; Alberts, A.S.; Gruber, H.E.
    Kinetic studies of mutant human adenylosuccinase (1989), Biochim. Biophys. Acta, 999, 19-23.
    View publication on PubMed
Search for more literature for 4.3.2.2

General Stability

General Stability Organism
dialysis for 24 h at 5°C, activity in mutant lysates is decreased by 95% while 50-60% of the initial activity remains in the normal cell lysates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00254
-
 N6-(1,2-dicarboxyethyl)AMP enzyme from cell line 1751 Homo sapiens
0.00289
-
 N6-(1,2-dicarboxyethyl)AMP enzyme from cell line 1753 Homo sapiens
0.0033
-
 N6-(1,2-dicarboxyethyl)AMP enzyme from wild-type cell lines Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 4 normal cell lines: 894, 1707, 1502 and 1454, 2 cell lines from patients with infantile autism: 1751, 1753
-

Source Tissue

Source Tissue Comment Organism Textmining
lymphoblast cultured Homo sapiens
-

Storage Stability

Storage Stability Organism
-70°C, 10% glycerol, 1 mM dithiothreitol, 0.01 M Tris, pH 7.4, activity in lysates from all cell lines is stable for more than 6 weeks Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 t1/2 for enzyme from mutant cell line 1751: 0.23 min, t1/2 for enzyme from mutant cell line 1753: 0.2 min, t1/2 for enzyme from the normal cell lines: 9.65 h Homo sapiens
60
-
 t1/2 for enzyme from mutant cell line 1751: 7.35 h, t1/2 for enzyme from mutant cell line 1753: 6.25 h, t1/2 for enzyme from the normal lines: 2.18 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 all cell lines examined Homo sapiens