Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
T78M | mutant enzyme with reduced catalytic activity | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X0C6 AND Q9X0C8 | Q9X0C6: subunit HisF, Q9X0C8: subunit HisH | - |
Thermotoga maritima ATCC 43589 | Q9X0C6 AND Q9X0C8 | Q9X0C6: subunit HisF, Q9X0C8: subunit HisH | - |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Subunits | Comment | Organism |
---|---|---|
dimer | the enzyme is a 1:1 complex of the glutaminase subunit HisH and the cyclase subunit HisF. Subunit HisF contains three distinct internal cavities, which can be identified by xenon-induced chemical shift changes of the neighboring amino acid residues. Two of these cavities are located at the active site at opposite ends of the substrate N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide binding groove. The third cavity is located in the interior of the central beta-barrel of HisF and overlaps with the putative ammonia transport channel | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
ImGP synthase | - |
Thermotoga maritima |