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Literature summary for 4.3.2.10 extracted from
- Expression and purification of imidazole glycerol phosphate synthase from Saccharomyces cerevisiae (2000), Protein Expr. Purif., 18, 366-377 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| catalytically active yeast HIS7 is expressed in Escherichia coli under the control of T7 polymerase promoter | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | pH 7.0, 30°C | Saccharomyces cerevisiae |  |
| 1.7 | - |
L-glutamine | pH 7.0, 30°C | Saccharomyces cerevisiae | |
| 246 | - |
NH4+ | pH 7.0, 30°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Saccharomyces cerevisiae |
| 57000 | - |
equilibrium sedimentation | Saccharomyces cerevisiae |
| 61000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
| 61003 | - |
MALDI MS | Saccharomyces cerevisiae |
| 61082 | - |
calculated from sequence | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | Saccharomyces cerevisiae | the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | Saccharomyces cerevisiae ATCC 204508 | the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P33734 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | P33734 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stored without loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | - |
Saccharomyces cerevisiae | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis | Saccharomyces cerevisiae | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | - |
Saccharomyces cerevisiae ATCC 204508 | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis | Saccharomyces cerevisiae ATCC 204508 | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+ | the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate | Saccharomyces cerevisiae | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O | - |
? | |
| 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+ | the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate | Saccharomyces cerevisiae ATCC 204508 | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 61000, SDS-PAGE | Saccharomyces cerevisiae |
| monomer | 1 * 61082, calculated from sequence | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HIS7 | - |
Saccharomyces cerevisiae |
| IGP synthase | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
NH4+ | pH 7.0, 30°C | Saccharomyces cerevisiae | |
| 3.9 | - |
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | pH 7.0, 30°C | Saccharomyces cerevisiae | |
| 5.2 | - |
L-glutamine | pH 7.0, 30°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
glutamine-dependent activity | Saccharomyces cerevisiae |
| 7 | 8.5 | NH4+-dependent activity | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis | Saccharomyces cerevisiae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
NH4+ | pH 7.0, 30°C | Saccharomyces cerevisiae | |
| 3.1 | - |
L-glutamine | pH 7.0, 30°C | Saccharomyces cerevisiae | |
| 950 | - |
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | pH 7.0, 30°C | Saccharomyces cerevisiae | |
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